Transgenic Mouse Methods and Protocols

Table of ContentsPrefaceContributors1. Introduction: Strategies for developing genetically modified miceMarten H. Hofker2. Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst InjectionJan Willem Vonken 3. Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryosJérôme Artus and Anna-Katerina Hadjantonakis4. Cryopreservation of mouse spermatozoa and In vitro fertilizationNaomi Nakagata5. Autopsy and histological analysis of the transgenic mouseMarion J.J. Gijbels and Menno P.J. de Winther6. Transgene designBart van de Sluis and Jan Willem Vonken7. Inducible Transgenic Mouse ModelsThomas L. Saunders8. Lentiviral transgenesisTerunaga Nakagawa and Casper C. Hoogenraad9. Transgenesis in Mouse Embryonic Stem CellsJanine van Ree, Wei Zhou, Ming Li and Jan van Deursen10. Engineering BAC Reporter Gene Constructs for Mouse Transgenesis Yu Fu and Peter Maye11. Targeting Vector Construction Through RecombineeringLiviu A. Malureanu12. Generating Conditional Knockout MiceRoland H. Friedel, Wolfgang Wurst, Benedikt Wefers and Ralf Kühn13. Hypomorphic miceDaren J. Baker14. MICER Targeting Vectors for Manipulating the Mouse GenomeChunhong Liu, Paul F. Szurek and Y. Eugene Yu15. Knockin approachesAnton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans16. Generation of a series of knockin alleles using RMCE in ES cells Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans17. Selection of targeted mutants from a library of randomly mutagenized ES cells Kyoji Horie, George Gaitanaris, and Alexander Gragerov18. Generation of genetically modified rodents using random ENU mutagenesisRuben van Boxtel and Edwin Cuppen19. Bone marrow transplantations to study gene function in hematopoietic cellsMenno P.J. de Winther and Peter Heeringa20. Adenovirus Mediated Gene TransferKo Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux and Jurre Hageman