Stress Response: Methods and Protocols

Part I. Detection and Assay of Stress-Induced Damage. Identifying and Counting Protein Modifications Triggered by Nitrosative Stress, Prabodh K. Sehajpal and Harry M. Lander. Determination of Carbonyl Groups in Oxidized Proteins, Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, and Emily Shacter. Quantitation of 4-Hydroxynonenal Protein Adducts, Koji Uchida and Earl R. Stadtman. Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate, Cecile M. Krejsa and Gary L. Schieven. The Measurement of Protein Degradation in Response to Oxidative Stress, Thomas Reinheckel, Tilman Grune, and Kelvin J. A. Davies. Part II. The Activation of Signal Transduction by Cellular Stress. Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress, D. Grahame Hardie, Ian P. Salt, and Stephen P. Davies. Detection and Activation of Stress-Responsive Tyrosine Kinases, Gary L. Schieven. Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells, Yamini Achari and Susan P. Lees-Miller. Expression and Assay of Recombinant ATM, Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, and Yosef Shiloh. Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells: IKK Signalsome, Frank Mercurio, David B. Young, and Anthony M. Manning. Methods to Assay Stress-Activated Protein Kinases, Ana Cuenda. Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators, Chao-Feng Zheng and Li Xu. Use of Kinase Inhibitors to Dissect Signaling Pathways, Ana Cuenda and Dario R. Alessi. The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation, Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, and Ted R. Hupp. Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs, D. Grahame Hardie. Part III. The Analysis of Stress-Induced Gene Expression. Assaying NF-kB and AP-1 DNA-Binding and Transcriptional Activity, Judith M. Mueller and Heike L. Pahl. Analysis of the Mammalian Heat Shock Response: Inducible Gene Expression and Heat Shock Factor Activity, Anu Mathew, Yanhong Shi, Caroline Jolly, and Richard I. Morimoto. Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression, Charareh Pourzand, Olivier Reelfs, and Rex M. Tyrrell. The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression, Michael W. Bate, Sushma R. Jassal, and David W. Brighty. SAGE: The Serial Analysis of Gene Expression, Jill Powell. Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol, Daniel J. Lavery, Phillippe Fonjallaz, Fabienne Fleury-Olela, and Ueli Schibler. UVB-Regulated Gene Expression in Human Keratinocytes: Analysis by Differential Display, Harry Frank Abts, Thomas Welss, Kai Breuhahn, and Thomas Ruzicka. Part IV. Analysis of Stress Protein Function. Heme Oxygenase Activity: Current Methods and Applications, Stefan W. Ryter, Egil Kvam, and Rex M. Tyrrell. Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches, Brian C. Freeman, Annamieke Michels, Jaewhan Song, Harm H. Kampinga, and Richard I. Morimoto. Analysis of Chaperone Properties of Small Hsp's, Monika Ehrnsperger, Matthias Gaestel, and Johannes Buchner. Analysis of Small Hsp Phosphorylation, Rainer Benndorf, Katrin Engel, and Matthias Gaestel. Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein by Tryptic Phosphopeptide Mapping, David W. Meek and Diane M. Milne. The Development of Physiological Models to Study Stress Protein Responses, Ted R. Hupp. Index.