Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Studies

Part I. Introduction and Reviews Calcium-Binding Proteins Hans J. Vogel, Richard D. Brokx, and Hui Ouyang Calcium Robert J. P. Williams Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, and John S. Elce The Multifunctional S100 Protein Family Claus W. Heizmann Ca2+ Binding to Proteins Containing g-Carboxyglutamic Acid Residues Egon Persson The Caseins of Milk as Calcium-Binding Proteins Harold M. Farrell, Jr., Thomas F. Kumosinski, Edyth L. Malin, and Eleanor M. Brown Part II. Calcium-Binding Proteins: Case Studies Preparation of Recombinant Plant Calmodulin Isoforms Raymond E. Zielinski Isolation of Recombinant Cardiac Troponin C John A. Putkey and Wen Liu Skeletal Muscle Troponin C: Expression and Purification of the Recombinant Intact Protein and Its Isolated N- and C-Domain Fragments Joyce R. Pearlstone and Lawrence B. Smillie Purification of Recombinant Calbindin D9k Eva Thulin S100 Proteins: From Purification to Functions Jean Christophe Deloulme, Gaëlh Ouengue Mbele, and Jacques Baudier Cadherins Jean-René Alattia, Kit I. Tong, Masatoshi Takeichi, and Mitsuhiko Ikura a-Lactalbumin and (Calcium-Binding) Lysozyme Katsutoshi Nitta Recombinant Annexin II Tetramer Hyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, and David M. Waisman Purification and Characterization of ALG-2: A Novel Apoptosis-Linked Ca2+-Binding Protein Mingjie Zhang and Kevin W.-H. Lo Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain V1 of Calpain Miroslaw Cygler, Pawel Grochulski, and Helen Blanchard Neurocalcin: Role in Neuronal Signaling Senadhi Vijay-Kumar and Vinod D.Kumar Crystallization and Structure-Function of Calsequestrin ChulHee Kang, William R. Trumble, and A. Keith Dunker Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains Eric A. Nalefski and Joseph J. Falke Ca2+-Binding Mode of the C2A-Domain of Synaptotagmin Josep Rizo, Josep Ubach, and Jesús García Study of Calcineurin Structure by Limited Proteolysis Seun-Ah Yang and Claude Klee