Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques

II. Methods to Study Calcium-Binding Proteins
Quantitative Analysis of Ca2+ Binding by Flow Dialysis
Michio Yazawa
Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators
Sara Linse
Deconvolution of Calcium Binding Curves: Facts and Fiction
Jacques Haiech and Marie-Claude Kilhoffer Absorption and Circular Dichroism Spectroscopy
Stephen R. Martin and Peter M. Bayley
Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins
Heinz Fabian and Hans J. Vogel
Steady State Fluorescence Spectroscopy
Aalim M. Weljie and Hans J. Vogel
Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins
J. David Johnson and Svetlana B. Tikunova
Surface Plasmon Resonance of Calcium-Binding Proteins
Karin Julenius
Differential Scanning Calorimetry
Maria M. Lopez and George I. Makhatadze
Isothermal Titration Calorimetry
Maria M. Lopez and George I. Makhatadze
Multiangle Laser Light Scattering and Sedimentation Equilibrium
Leslie D. Hicks, Jean-René Alattia, Mitsuhiko Ikura, and Cyril M. Kay
Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in Their Interactions with Regulatory Targets
Jill Trewhella and Joanna K. Krueger
Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry
Amanda L. Doherty-Kirby and Gilles A. Lajoie
Synthetic Calcium-Binding Peptides
Gary S. Shaw
Proteolytic Fragments of Calcium-Binding Proteins
Richard D. Brokx and Hans J. Vogel
Electron Magnetic Resonance Studies of Calcium-Binding Proteins
Lawrence J. Berliner
Cadmium-113 and Lead-207 NMR of Calcium-Binding Proteins
Teresa E. Clarke and Hans J. Vogel
Calcium-43 of NMR ofCalcium-Binding Proteins
Torbjörn Drakenberg
Exploring Familial Relationships Using Multiple Sequence Alignment
Aalim M. Weljie and Jaap Heringa
Structure Determination by NMR - Isotope Labeling
Monica X. Li, David C. Corson, and Brian D. Sykes
Protein Structure Calculation from NMR Data
Tapas K. Mal, Stefan Bagby, and Mitsuhiko Ikura
Shape and Dynamics of Calcium Binding Proteins Investigated by Nitrogen-15 NMR Relaxation
Jörn M. Werner, Iain D. Campbell, and A. Kristina Downing
The Use of Dipolar Couplings for the NMR Structure Refinement of a Pair of Calcium Binding EGF Domains
Jonathan Boyd, Iain D. Campbell, and A. Kristina Downing
Vector Geometry Mapping: A Method to Characterise the Conformation of Helix-Loop-Helix Calcium Binding Proteins
Kyoko L. Yap, James B. Ames, Mark B. Swindells, and Mitsuhiko Ikura
Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography
Ryoji Kobayashi
Enzymatic Assays to Compare Calmodulin Isoforms, Mutants and Chimeras
Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland, Ritsu Kondo, and J. David Johnson
Gene Expression in Transfected Cells
Kate Hughes, Juha Saarikettu, and Thomas Grundström
Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins
Anthony Persechini
Studying the Spatial Distribution of Ca2+-Binding Proteins: How Does it Work for Calmodulin?
Katalin Török, Richard Thorogate, and Steven Howell