Molecular Methods in Developmental Biology: Xenopus and Zebrafish

The Animal Cap Assay, Jeremy Green. Cell and Tissue Transplantation in Zebrafish Embryos, Toshiro Mizuno, Minori Shinya, and Hiroyuki Takeda. Ribonuclease Protection Analysis of Gene Expression in Xenopus, Craig S. Newman and Paul A. Krieg. Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), Oliver C. Steinbach and Ralph A. W. Rupp. Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos, Joanne Broadbent and E. Mary Read. In Situ Hybridization to Sections of Xenopus Embryos, David Bertwistle. Zebrafish Immunohistochemistry, Rachel Macdonald. Immunohistochemistry of Xenopus Embryos, Carl Robinson and Matthew Guille. Preparation and Testing of Synthetic mRNA for Microinjection, Wendy Moore and Matthew Guille. Microinjection into Xenopus Oocytes and Embryos, Matthew Guille. Microinjection into Zebrafish Embryos, Qiling Xu. Expression from DNA Injected into Xenopus Embryos, Ondine Cleaver and Paul A. Krieg. Promoter Analysis in Zebrafish Embryos, Jos Joore. Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs, Yuchang Fu, Donghui Kan, and Sylvia Evans. Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis, Rob Orford and Matthew Guille. DNA Footprinting Using Crude Embryo Extracts from Xenopus laevis, Rob Orford, Darren Mernagh, and Matthew Guille. Mapping Protein-DNA Interactions Using In Vivo Footprinting, David Warshawsky and Leo Miller. Index.