Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols

Part I. Techniques for Histological Identification Cytochemical Localization of H2O2 in Biological Tissues E. Ann Ellis and Maria B. Grant Localization of Intracellular Lipid Peroxides Using Tetramethylbenzidine Reaction for Transmission Electron Microscopy E. Ann Ellis, Shigehiro Iwabuchi, Don Samuelson, and Donald Armstrong The Immunohistochemical Localization of Glutathione Peroxidase Kiyoshi Akeo, Tadahisa Hiramitsu, and Keiichi Watanabe 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody Hideyuki J. Majima, Takako Nakanishi-Ueda, and Toshihiko Ozawa Immunohistochemical Detection of Protein Oxidation Jürgen Frank, Alfonso Pompella, and Hans K. Biesalski Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells Alfonso Pompella, Silvia Dominici, Jürgen Frank, and Hans K. Biesalski Part II. Techniques for Subcellular Localization Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts (AGE Receptors) Paul J. Thornalley Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes Kiyomi Kikugawa X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress R. Preston Mason and Robert F. Jacob Detection of Estrogen Receptor by In Situ Hybridization Kaori Kobayashi and Hiroshi Kobayashi Tumor Necrosis Factor-a Quantification and Expression by In Situ Hybridization Tracey A. Ignatowski and Robert N. Spengler Part III. Techniques for Molecular Biology Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells Ahmad Aljada, Husam Ghanim, and Paresh Dandona Activation of Nuclear Factor-kB (NF-kB) in Mononuclear Cells (MNC) Ahmad Aljada, Husam Ghanim, and PareshDandona Assay of Intracellular Hydrogen Peroxide Generation in Activated Individual Neutrophils by Flow Cytometry Yoshikazu Ito and David A. Lipschitz DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs) Gieri Camenisch, Roland H. Wenger, and Max Gassmann Analysis of Heat-Shock Transcription Factor and Element-Binding Activity Yong J. Lee Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation Enbo Ma and Gabriel G. Haddad An Expression System for a Transporter of Iron and Other Metals Michael D. Garrick and Kevin G. Dolan Analysis of Gene Expression Following Oxidative Stress Dana R. Crawford, Toshihide Suzuki, Jan Sesay, and Kelvin J. A. Davies Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis Andrew Chow, Shahid Ahmed, Larrisa Chaplia, and Joseph Mattana Thioredoxin and Redox Regulation of the Nuclear Receptor Yuichi Makino, Kensaku Okamoto, and Hirotoshi Tanaka Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA Xin Gen Lei and Wen-Hsing Cheng Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase Kunio Yagi, Sadaaki Komura, and Nobuko Ohishi Part IV. Techniques for In Vivo/Ex Vivo/In Vitro Applications Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method Takashi Sakurai and Susumu Terakawa In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy David M. Hall and Garry R. Buettner Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHz Jürgen Fuchs, Norbert Groth, and Thomas Herrling In Vivo/In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy Pei Tsai, Supatra Porasuphatana, Howard J. Halpern,