E. coli: Shiga Toxin Methods and Protocols

The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections: An Overview
Mohamed A. Karmali
Methods for Detection of STEC in Humans: An Overview
James C. Paton and Adrienne W. Paton
Serological Methods for the Detection of STEC Infections
Martin Bitzan and Helge Karch
Detection and Characterization of STEC in Stool Samples Using PCR
Adrienne W. Paton and James C. Paton
Molecular Typing Methods for STEC
Haruo Watanabe, Jun Terajima, Hidemasa Izumiya, and Sunao Iyoda
STEC in the Food Chain: Methods for Detection of STEC in Food Samples
Michael Bülte
STEC as a Veterinary Problem: Diagnostics and Prophylaxis in Animals
Lothar H. Wieler and Rolf Bauerfeind
Cellular Microbiology of STEC Infections: An Overview Frank Ebel and Dana Philpott
Analysis of Pathogenicity Islands of STEC
Tobias A. Oelschlaeger, Ulrich Dobrindt, Britta Janke, Barbara Middendorf, Helge Karch, and Jörg Hacker
Generation of Isogenic Deletion Mutants of STEC
Soudabeh Djafari, Nadja D. Hauf, and Judith F. Tyczka
Generation of Monoclonal Antibodies Against Secreted Proteins of STEC
Kirsten Niebuhr and Frank Ebel
Microscopic Methods to Study STEC: Analysis of the Attaching and Effacing Process
Stuart Knutton
Detection and Characterization of EHEC-Hemolysin
Herbert Schmidt and Roland Benz
Shiga Toxin Receptor Glycolipid Binding: Pathology and Utility
Clifford A. Lingwood
Methods for the Purification of Shiga Toxin 1
Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood
Methods for the Identification of Host Receptors for Shiga Toxin
Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood
Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
Frédéric Mallard and Ludger Johannes
Measuring pHWithin the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin
Jae H. Kim
Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways
Nicola L. Jones
Interaction of Shiga Toxin with Endothelial Cells
Martin Bitzan and D. Maroeska W. M. te Loo
Shiga Toxin Interactions with the Intestinal Epithelium
Cheleste M. Thorpe, Bryan P. Hurley, and David W. K. Acheson
Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes
Christian Menge
Animal Models for STEC-Mediated Disease
Angela R. Melton-Celsa and Alison D. O'Brien
Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC
Florian Gunzer, Isabel Hennig-Pauka, Karl-Heinz Waldmann, and Michael Mengel
Bovine Escherichia coli O157: H7 Infection Model
Evelyn A. Dean-Nystrom
Index