Molecular Motors: Methods and Protocols

ContentsPrefaceContributors CH. 1 Equilibrium Binding of Proteins to F-Actin, Joseph M. ChalovichCH. 2 Analysis of Calcium/Calmodulin Regulation of a Plant KinesinUsing Cosedimentation and ATPase Assays, Anireddy S.N. Reddy CH. 3 In Vitro and In Vivo Analysis of Microtubule DestabilizingKinesins, Jason Stumpff, Jeremy Cooper, Sarah Domnitz, Ayana Moore,Kathleen Rankin, Mike Wagenbach and Linda Wordeman CH. 4 Approaches to Kinesin-1 Phosphorylation, Gerardo Morfini, GustavoPigino, and Scott T. Brady CH. 5 Protein Modification to Probe Intra-dynein Interactions and InVivo Redox State, Ken-ichi Wakabayashi, Miho Sakato and Stephen M.King CH. 6 Methods to Study the Interactions of the Dynein Light Chains andIntermediate Chains, Kevin W.-H. Lo and K. Kevin Pfister CH. 7 Identification of Motor Protein Cargo by Yeast 2-Hybrid AndAffinity Approaches, Yuguo Zhang, Rong Wang, Holly Jefferson, and AnnO. Sperry CH. 8 In Situ Binding Assay to Measure Myosin-1c Interactions withHair-Cell Proteins, Kelli R. Phillips and Janet L. Cyr CH. 9 Ultrastructural Analysis of Kinesin-related Motor Proteins DuringSpermatogenesis, Wan-Xi Yang CH. 10 In Vitro Motility System to Study the Role of Motor Proteins inReceptor-Ligand Sorting, John W. Murray and Allan W. Wolkoff CH. 11 Enrichment and Disassembly of Ectoplasmic Specializations in theRat Testis, Julian A. Guttman, Kuljeet S.Vaid and A. Wayne VoglCH. 12 Single-Molecule Observation of Rotation of F1-ATPase throughMicro Beads, Takayuki Nishizaka, Kana Mizutani and Tomoko Masaike CH. 13 The use of FRET in the Analysis of Motor Protein Structure,Andre Kasprzak CH. 14 Structure Determination of the Motor Domain of Yeast KinesinKar3 by X-ray Crystallography, Hee-Won Park CH. 15 High Resolution Structural Analysis of the Kinesin-MicrotubuleComplex by Electron Cryo-Microscopy, Keiko Hirose and LindaA. Amos CH. 16 Chemical-Genetic Inhibition of Sensitized Mutant UnconventionalMyosins, Ryan L. Karcher, D. William Provance, Jr., Peter G. Gillespie,and John A. Mercer