Adipose Tissue Protocols

Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders Gema Frühbeck Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity Louis Casteilla, Luc Pénicaud, Béatrice Cousin, and Denis Calise Application of Imaging and Other Non-invasive Techniques in Determining Adipose Tissue Mass Wei Shen and Jun Chen Generation of Adipose Tissue-Specific Transgenic Mouse Models Xian-Cheng Jiang Angiogenesis in Adipose Tissue Ebba Brakenhielm and Yihai Cao Adipose Organ Nerves Revealed by Immunohistochemistry Antonio Giordano, Andrea Frontini and Saverio Cinti Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow Gijs H. Goossens and Fredrik Karpe Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues Barbara Cannon and Jan Nedergaard Application of Lipidomics and Metabolomics to the Study of Adipose Tissue Ismo Mattila, Tuulikki Seppänen-Laakso, Tapani Suortti, and Matej Oresic Applications of Proteomics to the Study of Adipose Tissue Sonja Hess and Xiaoli Chen Application of DNA Microarray to the Study of Human Adipose Tissue/Cells Paska A. Permana, Saraswathy Nair, and Yong-Ho Lee Differentiation of Adipose Stem Cells Bruce A. Bunnell, Bradley T. Estes, Farshid Guilak, and Jeffrey M. Gimble Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells Colin R Jefcoate, Suqing Wang and Xueqing Liu Explant Cultures of White Adipose Tissue Sébastien Thalmann, Cristiana E.Juge-Aubry and Christoph A. Meier Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue Dorothy B. Hausman, Hea Jin Park and Gary J. Hausman Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue Danett K. Brake and C. Wayne Smith Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation to the Study of Transcription in Adipose Cells Melina M. Musri, Ramon Gomis and Marcelina Párrizas Application of RNAi Techniques to Adipose Cell Cultures Shigeki Shimba RNA Isolation and Real-time Quantitative RT-PCR Haiyan Guan and Kaiping Yang Study of Adipose TissueGene Expression by In Situ Hybridization Michel Grino Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures Daniela Malide Determination of Lipolysis in Isolated Primary Adipocytes Srikant Viswanadha and Constantine Londos Study of Glucose Uptake in Adipose Cells Jun Shi and Konstantin V. Kandror Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction Alexander Sorisky and AnneMarie Gagnon