Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols

1. Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy ApplicationsGuillermo Guenechea, Jose C. Segovia, and Juan A. Bueren2. Isolation of Human and Mouse Hematopoietic Stem CellsYuk Yin Ng, Miranda R.M. Baert, Edwin F.E. de Haas, Karin Pike-Overzet, and Frank J.T. Staal3. Murine Hematopoietic Stem Cell Transduction Using Retroviral VectorsUte Modlich, Axel Schambach, Zhixiong Li, and Bernhard Schiedlmeier4. Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-Mediated Gene Transfer for Clinical Trials Tulin Budak-Alpdogan and Isabelle Rivière5. Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer Francesca Santoni Di Sio and Luigi Nalidini6. T Cell Culture for Gammaretroviral Transfer Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer7. Retroviral Transduction of Murine Primary T LymphocytesJames Lee, Michel Sadelain, and Renier Brentjens8. Lentiviral Vector Gene Transfer into Human T CellsEls Verhoeyen, Caroline Costa, and Francois-Loic Cosset9. DNA Transposons for Modification of Human Primary T LymphocytesXin Huang, Andrew C. Wilber, R. Scott McIvor, and Xianzheng Zhou10. Retroviral Gene Transfer into Primary Human Natural Killer CellsEvren Alici, Tolga Sutlu, and M. Sirac Dilber11. Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic CellsRenata Stripecke12. In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem CellsDao Pan13. In-vivo and ex-vivo Gene Transfer in Thymocytes and Thymocyte-PrecursorsOumeya Adjali, Amélie Montel-Hagen, Louise Swainson, Sophie Marty, Rita Vicente, Cedric Mongellaz, Chantal Jacquet, Valérie Zimmermann, andNaomi Taylor14. Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic CellsAxel Schambach, William S. Swaney, and Johannes C. M. van der Loo15. Knock-Down of Gene Expression in Hematopoietic CellsMichaela Scherr, Letizia Venturini, and Matthias Eder16. The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell PopulationsRainer Löw 17. Detection of Replication Competent Retrovirus and Lentivirus Lakshmi Sastry and Kenneth Cornetta18. Release Testing of Retroviral Vectors and Gene Modified CellsDiana Nordling, Anne Kaiser, and Lilith Reeves19. Copy Number Determination of Genetically Modified Hematopoietic Stem CellsTodd Schuesler, Lilith Reeves, Christof von Kalle, and Elke Grassman20. Tissue Procurement for Molecular Studies Using Laser-Assisted MicrodissectionUlrich Lehmann and Hans Kreipe21. Leukemia Diagnosis in Murine Bone Marrow Transplantation Models Zhixiong Li, Ute Modlich, and Anjali Mishra22. Humanized Mouse Models to Study the Human Haematopoietic Stem Cell CompartmentDominique Bonnet23. Canine Models of Gene-Modified HematopoiesisBrian C. Beard and Hans-Peter Kiem24. Detection of Retroviral Integration Sites by Linear Amplification Mediated PCR (LAM-PCR) and Tracking of Individual Integration Clones in Different SamplesManfred Schmidt, Kerstin Schwarzwaelder, Cynthia Bartholomae, Hanno Glimm, and Christof von Kalle25. Retroviral Insertion Site Analysis in Dominant Haematopoietic ClonesOlga S. Kustikova, Ute Modlich, and Boris Fehse26. Tracking Gene-Modified T-Cells In vivoAlessandra Recchia and Fulvio Mavilio27. DNA Microarray Studies of Hematopoietic