Plant Embryo Culture: Methods and Protocols

Part I: Culture of Zygotic Embryos: Fundamental Studies

1. Zygotic Embryo Culture: An Overview

Tegan M. Haslam and Edward C. Yeung

2. In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture

Takashi Okamoto

3. Canola Zygotic Embryo Culture

Nicole S. Ramesar-Fortner and Edward C. Yeung

4. Immature Seeds and Embryos of Medicago truncatula Cultured In vitro

Sergio J. Ochatt

5. In vitro Culture and Germination of Terrestrial Asian Orchid Seeds

Yung-I Lee

6. In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos

Florent Engelmann, Bernard Malaurie, and Oulo N'Nan

Part II: Application of Embryo Culture Techniques: Embryo Rescue

7. Immature Embryo Rescue and Culture

Xiuli Shen, Fred G. Gmitter, Jr., and Jude W. Grosser

8. Chickpea Hybridization Using In vitro Techniques

Nalini Mallikarjuna and Fred J. Muehlbauer

9. Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture

Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón, and Neftalí Ochoa-Alejo

10. Phaseolus Immature Embryo Rescue Technology

Pascal Geerts, André Toussaint, Guy Mergeai, and Jean-Pierre Baudoin

11. Wide Crossing in Lentil through Embryo Rescue

Richard Fratini and Maria L. Ruiz

12. Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques

Ajoy Kumar Roy, Devendra Ram Malaviya, and Pankaj Kaushal

Part III: Cryopreservation of Embryos

13. Cryopreservation of Embryos: An Overview

Florent Engelmann

14. Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm

Anna De Carlo, Maurizio Lambardi, and Elif Aylin Ozudogru

15. Cryopreservation of Zygotic Embryo Axes and Somatic Embryos of European Chestnut

Ana M. Vieitez, M. Carmen San-José, and Elena Corredoira

16. Cryopreservation of Ilex Immature Zygotic Embryos

Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, and Hebe Rey

Part IV: The Use of Zygotic Embryos as Explants for Somatic Embryogenesis and/or Organogenesis

17. The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview

Mohamed Elhiti and Claudio Stasolla

18. Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos

Malgorzata D. Gaj

19. Pine Somatic Embryogenesis Using Zygotic Embryos as Explants

Gerald S. Pullman and Kylie Bucalo

20. Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies

Kee Yoeup Paek, Eun Joo Hahn, and So Young Park

Part V: Transformation Using Zygotic Embryos as Explants

21. Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview

Muhammad Tahir, Ejaz A. Waraich, and Claudio Stasolla

22. Genetic Transformation Using Maize Immature Zygotic Embryos

Bronwyn Frame, Marcy Main, Rosemarie Schick, and Kan Wang

23. Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue

Martha M. O'Kennedy, Hester C. Stark, and Nosisa Dube

24. Agrobacterium tumefaciens-Mediated Genetic Transformation of Cereals Using Immature Embryos

Ashok K. Shrawat and Allen G. Good