Telomeres and Telomerase: Methods and Protocols

1. Introduction to Telomeres and Telomerase

Zhou Songyang

2. Analysis of Average Telomere Length in Cultured Human Cells

Dan Liu

3. Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH)

Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo

4. Telomere Strand-Specific Length Analysis by Fluorescent In situ Hybridization (Q-CO-FISH)

Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo

5. Telomere G-Overhang Length Measurement Method 1: The DSN Method

Yong Zhao, Jerry W. Shay, and Woodring E. Wright

6. Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA

Hidetoshi Tahara

7. Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-rich Fill-In

Yong Zhao, Jerry W. Shay, and Woodring E. Wright

8. G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

Raveendra I. Mathad and Danzhou Yang

9. Analysis of Yeast Telomerase by Primer Extension Assays

Min Hsu and Neal F. Lue

10. Telomeric Repeat Amplification Protocol (TRAP): Measuring the Activity of the Telomerase

Huawei Xin

11. CO-FISH, COD-FISH, ReD-FISH, SKY-FISH

Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin, and Susan M. Bailey

12. Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques

Eladio Abreu, Rebecca M. Terns, and Michael P. Terns

13. Cytogenetic Analysis of Telomere Dysfunction

Asha Multani and Sandy Chang

14. Probing the Telomere Damage Response

Relcha Rai and Sandy Chang

15. Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)

Wenbin Ma

16. Studying of Telomeric Protein-Protein Interactions by Bi-Molecular Fluorescence Complementation (BiFC) and Peptide Array-Based Assays

Wenbin Ma, Hyeung Kim, and Zhou Songyang

17. Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation

Feng Wang and Ming Lei