Protein Expression in Mammalian Cells: Methods and Protocols

1. Why Proteins in Mammalian Cells?

James L. Hartley

2. Large Scale Transfection of Mammalian Cells

Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm

3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters

Christine T. DeMaria

4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles

Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash

5. Transfection of Difficult-to-Transfect Primary Mammalian Cells

Oliver Gresch and Ludger Altrogge

6. Stable Protein Expression in Mammalian Cells Using Baculoviruses

Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch

7. Using Matrix Attachment Regions to Improve Recombinant Protein Production

Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod

8. Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells

Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald

9. Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells

Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang

10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells

Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens

11. Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker

Say Kong Ng

12. Tools for Co-Producing Multiple Proteins in Mammalian Cells

Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia

13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases

Paula Magnelli, Alicia Bielik, and Ellen Guthrie

14. Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA

Renate Kunert and Karola Vorauer-Uhl

15. Methods for Constructing Clones for Protein Expression in Mammalian Cells

Takefumi Sone and Fumio Imamoto

16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells

Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito