Mitochondrial Bioenergetics: Methods and Protocols

1 - Overview of Mitochondrial Bioenergetics (Vitor M. C. Madeira)

2 - Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells (Ana M. Silva and Paulo J. Oliveira)

3 - High-Resolution Respirometry. OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle (Dominik Pesta and Erich Gnaiger)

4 - High Throughput Analysis of Mitochondrial Oxygen Consumption (James Hynes, Rachel L. Swiss and Yvonne Will)

5 - Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices (Ana Ledo, Rui M. Barbosa and João Laranjinha)

6 - Mitochondrial Membrane Potential ( D Y ) Fluctuations Associated with the Metabolic States of Mitochondria (Carlos M. Palmeira and Anabela P. Rolo)

7 - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells (Tiago R. Figueira, Daniela R. Melo, Aníbal E. Vercesi and Roger F. Castilho)

8 - Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells (D. Nicholls)

9 - Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria (Vilmante Borutaite and Rasa Baniene)

10 - Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function (Martin Jastroch)

11 - Measurement of Proton Leak and Electron Leak in Isolated Mitochondria

(Charles Affourtit, Casey L. Quinlan and Martin D. Brand)

12 - Relation between Mitochondrial Membrane Potential and ROS Formation (Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski and Mariusz R. Wieckowski)

13 - Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport (António J. Moreno and Joaquim A. F. Vicente)

14 - Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy (Sean M. Davidson and Michael R. Duchen)

15 - Mitochondrial Permeability Transition Pore and Calcium Handling

(Renee Wong, Charles Steenbergen and Elizabeth Murphy)

16- Imaging of Mitochondrial pH Using SNARF-1

(Venkat K. Ramshesh and John J. Lemasters)

17- Redox Equivalents and Mitochondrial Bioenergetics

(James R. Roede, Young-Mi Go and Dean P. Jones)

18-NMR Methodologies for Studying Mitochondrial Bioenergetics

(Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)

19-Computational Modeling of Mitochondrial Function

(Sonia Cortassa and Miguel A. Aon)