Two Hybrid Technologies: Methods and Protocols

1. Matrix-Based Yeast Two-Hybrid Screen Strategies and Comparison of Systems

Roman Häuser, Thorsten Stellberger, Seesandra V. Rajagopala, and Peter Uetz

2. Array-Based Yeast Two-Hybrid Screens: A Practical Guide

Roman Häuser, Thorsten Stellberger, Seesandra V. Rajagopala, and Peter Uetz

3. High Throughput Yeast Two-Hybrid Screening

George G. Roberts III, Jodi R. Parrish, Bernardo A. Mangiola, and Russell L. Finley Jr.

4. A Stringent Yeast Two-Hybrid Matrix Screening Approach for Protein-Protein Interaction Discovery

Josephine Worseck, Arndt Grossmann, Mareike Weimann, Anna Hegele, and Ulrich Stelzl

5. High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries

Kerstin Mohr and Manfred Koegl

6. Virus-Human Cell Interactomes

Lionel Tafforeau, Chantal Rabourdin-Combe and Vincent Lotteau

7. Interactome Mapping in Malaria Parasites: Challenges and Opportunities

Douglas J. LaCount

8. Mapping Interactomes with High Coverage and Efficiency Using the Shifted Transversal Design

Xiaofeng Xin, Charles Boone, and Nicolas Thierry-Mieg

9. Assigning Confidence Scores for Protein-Protein Interactions

Jingkai Yu, Thilakam Murali, and Russell L. Finley Jr.

10. The Intergration and Annotation of the Human Interactome in the UniHI Database

Gautam Chaurasia and Matthias Futschik

11. Gene-Centered Yeast One-Hybrid Assays

John S. Reece-Hoyes and Albertha J.M. Walhout

12. One- plus Two-hybrid System for the Efficient Selection of Missense Mutant Alleles Defective in Protein-Protein Interactions

Ji Young Kim, Ok Gu Park, and Young Chul Lee

13. Investigation of Membrane Protein Interactions Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System

Julia Petschnigg, Victoria Wong, Jamie Snider, and Igor Stagljar

14. Application of the Split-Protein Sensor Trp1 to Protein Interaction Discovery in the Yeast Saccharomyces cerevisiae

Mandana Rezwan, Nicolas Lentze, Lukas Baumann and Daniel Auerbach

15. Tetracycline Repressor-Based Mammalian Two-Hybrid Systems

Kathryn Moncivais and Zhiwen Jonathan Zhang

16. The Fluorescent Two-Hybrid (F2H) Assay for Direct Analysis of Protein-Protein Interactions in Living Cells

Kourosh Zolghadr, Ulrich Rothbauer and Heinrich Leonhardt

17. ArrayMAPPIT: a Screening Platform for Human Protein Interactome Analysis

Sam Lievens, Nele Vanderroost, Dieter Defever, José Van der Heyden and Jan Tavernier

18. MAPPIT as a High-Throughput Screening Assay for Modulators of Protein-Protein Interactions in HIV and HCV

Bertrand Van Schoubroeck, Koen Van Acker, Géry Dams, Dirk Jochmans, Reginald Clayton, Jan Martin Berke, Sam Lievens, José Van der Heyden and Jan Tavernier

19. Integrated Measurement of Split TEV and cis-Regulatory Assays Using EXT Encoded Reporter Libraries

Anna Botvinik and Moritz J. Rossner