Mucins: Methods and Protocols

1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins

Karine Rousseau and Dallas M. Swallow

2. Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies

Julia R. Davies, Claes Wickström and David J. Thornton

3. Detecting, Visualising and Quantifying Mucins

Ceri A. Harrop, David J. Thornton and Michael A. McGuckin

4. Mass Spectrometric Analysis of Mucin Core Proteins

Mehmet Kesimer and John K. Sheehan

5. O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates

Thomas A. Gerken

6. Analysis of Assembly of Secreted Mucins

Malin E.V. Johansson and Gunnar C. Hansson

7. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes

Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey

8.Glycomic Work-flow for Analysis of Mucin O -linked Oligosaccharides

Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunseng Jin, and Niclas G. Karlsson

9. O-Glycomics: Profoliling and Structural Analysis of Mucin-type O-linked Glycans

Isabelle Breloy

10. O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry

Franz-Georg Hanisch

11. Analysing Mucin Degradation
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

12. Assessment of Mucus Thickness and Production In Situ

Lena Holm and Mia Phillipson

13. Preservation of Mucus in Histrological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH

Malin E.V. Johansson and Gunnar C. Hansson

14. In vitro Measurements of Mucus Secretion by Colon Explants

Jenny K. Gustafsson, Henrik Sjövall and Gunnar C. Hansson

15. Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function
David R. Hill and Brian Button

David R. Hill and Brian Button

David R. Hill and Brian Button

David R. Hill and Brian Button

16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K. Sheehan, and C. William Davis

17. Assessment of Intracellular Mucin Content In Vivo

Lucia Piccotti, Burton F. Dickey, Christopher M. Evans

18. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo

Yong H. Sheng, Sumaira Z. Hasain, Chin Wen Png, Michael A. McGuckin and Sara K. Linden

19. Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro

Pablo Augueso and Ilene K. Gipson

16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K.