Transgenic Plants: Methods and Protocols

Contents

Preface

Contributors

Part I. Selection AND DETECTION methods

1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation

Craig M. Richael and Caius M. Rommens

2. Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed

T. Scott Pinkerton, James R. Wild, and John A. Howard

3. Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants

Basel Khraiwesh

Part II. Transformation Technology - Lower Plants

4. Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock

Part III. Transformation Technology - Rice

5. A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)

Kenjirou Ozawa

6. Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene

Masaki Endo, Tsutomu Shimizu and Seiichi Toki

7. Visual Selection in Rice-a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

Hiroaki Saika, Haruko Onodera and Seiichi Toki

8. Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System

Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui

9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments

Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui

Part IV. Transformation Technology - Cereals and Other Monocots

10. Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos

Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux

11. Split-transgene Expression in Wheat

Mario Gils, Myroslava Rubtsova, and Katja Kempe

12. Agrobacterium-mediated Transformation of Brachypodium Distachyon

Vera Thole and Philippe Vain

13. Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules

Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm

14. Biolistic Mediated Production of Transgenic Oil Palm

Ghulam Kadir Ahmad Parveez and Bohari Bahariah

15. Transformation of Oil Palm using Agrobacterium tumefaciens

Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani

Part V. Transformation Technology - Dicots

16. Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)

César Petri, Ralph Scorza, and Chinnathambi Srinivasan

17. Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes

Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray

18. Initiation and Transformation of Grapevine Embryogenic Cultures

Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray

19. Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless

Mercedes Dabauza and Leonardo Velasco

20. Cotton Pistil Drip Transformation Method

Tianzhen Zhang and Tianzhi Chen

21. Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton

Tian-zhen Zhang and Shen-jie Wu

22. Targeted Biolistics for Improved Transformation of Impatiens balsamina

Andy Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi

23. A Protocol for Transformation of Torenia

Ryutaro Aida

24. Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia

Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi, and Tomoko Abe

Part VI. Gene Targeting, Silencing and Directed Mutation

25.

Isam Fattash, Basel Khraiwesh , M. Asif Arif and Wolfgang Frank

26. High Frequency of Single-copy T-DNA Transformants Produced after Floral Dip in CRE-expressing Arabidopsis Plants

Annelies De Paepe, Sylvie De Buck, Jonah Nolf, and Ann Depicker

27. A Developmentally Regulated Cre-lox system to Generate Marker-free Transgenic Brassica napus Plants

Lilya Kopertekh, Inge Broer, and Joachim Schiemann

28. Exploiting Multisite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic EngineeringLeandro H. Estornell, Antonio Granell, and Diego Orzaez

29. A One-time Inducible Transposon to Create Knockout Mutants in Rice

Yuh-Chyang Charng

30. Marker-free Gene Targeting by Recombinase-mediated Cassette Exchange (RMCE)

Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine

31. Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases

Tonya L. Strange Moynahan and Joseph F. Petolino

32. Double-strand Break-induced Targeted Mutagenesis in Plants

L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, and Spencer Jones

Part VII. Metabolic EnginEering and Pharming

33. Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway

Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera, Ramon Canela, Gerhard Sandman, Richard M. Twyman, Teresa Capell, Changfu Zhu, and Paul Christou

34. Production of a His-Tagged Canecystatin in Transgenic Sugarcane

Flavio Henrique-Silva and Andrea Soares-Costa

35. Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals

Nunzia Scotti and Teodoro Cardi

36. Use of a Callus-Specific Selection System to Development Transgenic Rice Seed Accumulating a High Level of Recombinant Protein

Yuhya Wakasa and Fumio Takaiwa

Part VIII. Field TESTS

37. How to Grow Transgenic Arabidopsis in the Field

Hanna Johansson Jänkänpää and Stefan Jansson