Chemotaxis: Methods and Protocols

1. Microfluidic Techniques for the Analysis of Bacterial ChemotaxisDerek L. Englert, Arul Jayaraman, and Michael D. Manson2. Prokaryotic PhototaxisWouter D. Hoff, Michael A. van der Horst, Clara B. Nudel, and Klaas J. Hellingwerf3. Photoorientation in Photosynthetic FlagellatesDonat-Peter Häder and Michael Lebert4. Dictyostelium Slug PhototaxisSarah J. Annesley and Paul R. Fisher5. Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell MigrationGuangping Tai, Brian Reid, Lin Cao, and Min Zhao6. Chemotropism During Yeast MatingPeter J. Follette and Robert A. Arkowitz7. Group Migration and Signal Relay In DictyosteliumPaul W. Kriebel and Carole A. Parent8. Quantitative Analysis of Distal Tip Cell Migration in C. elegansMyeongwoo Lee and Erin J. Cram9. Inflammation and Wound Healing in DrosophilaBrian Stramer and Will Wood10. Neutrophil Motility In Vivo Using ZebrafishJonathan R. Mathias, Kevin B. Walters, and Anna Huttenlocher11. Chemotaxis in Neutrophil-like HL-60 CellsArthur Millius and Orion D. Weiner12. Chemokine Receptor Dimerization and ChemotaxisJosé Miguel Rodríguez-Frade, Laura Martinez Muñoz, Borja L. Holgado, and Mario Mellado13. Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph NodesChung Park, Il-Young Hwang, and John H. Kehrl14. Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM MicroscopyXuehua Xu, Peter Johnson, and Susette C. Mueller15. In vivo Assay for Tumor Cell InvasionLorena Hernandez, Tatiana Smirnova, Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall16. Quantitative Studiesof Neuronal Chemotaxis in 3DWilliam J. Rosoff, Ryan G. McAllister, Geoffrey J. Goodhill, and Jeffrey S. Urbach17. Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylation in DictyosteliumYoichiro Kamimura, Ming Tang, and Peter Devreotes18. Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase ActivationXin-Hua Liao and Alan R. Kimmel19. Quantifying In Vivo Phosphoinositide Turnover in Chemotactically-Competent Dictyostelium CellsNadine Pawolleck and Robin S.B. Williams20. In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideumClaire Y. Allan and Paul R. Fisher21. Chemokine Receptor Signaling and HIV Infection Yuntao Wu22. Spatiotemporal Stimulation of Single Cells Using Flow PhotolysisCarsten Beta23. Spatio-Temporal Regulation of Ras-GTPases During Chemotaxis Atsuo T. Sasaki and Richard A. Firtel24. FRAP Analysis of Chemosensory Components of DictyosteliumCarrie A. Elzie and Chris Janetopoulos25. Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging and Single Molecule ImagingXuehua Xu, Joseph A. Brzostowski, and Tian Jin26. Imaging Actin Cytoskeleton Dynamics in Dictyostelium ChemotaxisGünther Gerisch27. Analysis of Actin Assembly by In Vitro TIRF MicroscopyDennis Breitsprecher, Antje K. Kiesewetter, Joern Linkner, and Jan Faix28. Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium CellsYukihiro Miyanaga, Satomi Matsuoka, and Masahiro Ueda29. Imaging B-cell Receptor Signaling by Single Molecule TechniquesPavel Tolar and Tobias