Epithelial Cell Culture Protocols: Second Edition

1. Progress Towards Drosophila Epithelial Cell Culture

Amanda Simcox

2. Culture of Choriod Plexus Epithelial Cells and In Vitro Model of Blood-CSF Barrier

Andrew D. Monnot and Wei Zheng

3. Methods for Culture of Human Corneal and Conjunctival Epithelia

Sandra J. Spurr-Michaud and Ilene K. Gipson

4. The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye

Timothy A. Blenkinsop, Enrique Salero, Jeffrey Stern, and Sally Temple

5. Oral and Pharyngeal Epithelial Keratinocyte Culture

Kantima Leelahavanichkul and J. Silvio Gutkind

6. Primary Culture of the Human Olfactory Neuroepithelium

Nancy E. Rawson and Mehmet Hakan Ozdener

7. Primary Culture of Mammalian Taste Epithelium

Mehmet Hakan Ozdener and Nancy E. Rawson

8. Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture

M. Leslie Fulcher and Scott H. Randell

9. Culture and Differentiation of Mouse Tracheal Epithelial Cells

Yingjian You and Steven L. Brody

10. Isolation and Culture of Alveolar Epithelial Type I and Type II cells from Rat Lungs

Robert F. Gonzalez and Leland G. Dobbs

11. Classical Human Epidermal Keratinocyte Cell Culture

Cathy Rasmussen, Christina Thomas-Virnig, and B. Lynn Allen-Hoffmann

12. Mouse Epidermal Keratinocyte Culture

Luowei Li

13. Three Dimensional Culture of Human Breast Epithelial Cells: The How and The Why

Pierre-Alexandre Vidi, Mina J. Bissell, and Sophie A. Lelièvre

14. Three Dimensional Cultures of Mouse Mammary Epithelial Cells

Rana Mroue and Mina J. Bissell

15. Isolation, Characterisation and Re-aggregate Culture of Thymic Epithelial Cells

Natalie Seach, Maree Hammett, and Ann Chidgey

16. Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development

Akihide Kamiya and Hiromitsu Nakauchi

17. Long Term Culture and Co-Culture of Primary Rat and Human Hepatocytes

Maria Shulman and Yaakov Nahmias

18. Tissue-Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidney Disease

Yasuni Nakanuma, Yasunoi Sato, and Kenichi Harada

19. Primary Mouse Small Intestinal Epithelia Cell Cultures

Toshiro Sato and Hans Clevers

20. Isolation, Growth and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Methods

J.J. Gildea, H.E. McGrath, R. E. VanSciver, Wang D. Bigler, and Robin A. Felder

21. Human Female Reproductive Tract Epithelial Cell Culture

Rachel W. S. Chan, Abby S. C. Mak, William S. B. Yeung, Kai-Fai Lee, Annie N. Y. Cheung, Hextan Y. S. Ngan, and Alice S. T. Wong

22. Primary Culture and Propagation of Human Prostate Epithelial Cells

Birunthi Niranjan, Mitchell G. Lawrence, Melissa M. Papargiris, Michelle G. Richards, Shirin Hussain, Mark Frydenberg, John Pedersen, Australian Prostate Cancer BioResource, Renea A. Taylor, and Gail P. Risbridger

23. Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Structures

Yuanyuan Zhang and Anthony Atala

24. Analysis of Tissue Interactions in Ectodermal Organ Culture

Pauliina M. Munne, Katja Närhi, and Frederic Michon

25. Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering

Patrick Salmon

26. Viral Transformation of Epithelial Cells