Pluripotent Stem Cells: Methods and Protocols

Part I. Generation of Pluripotent Stem Cells

1. Sources and Derivation of Human Embryonic Stem Cells

Michal Amit

2. A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells

Hossein Baharvand and Seyedeh-Nafiseh Hassani

3. A Review of the Methods for Human iPSC Derivation

Nasir Malik and Mahendra S. Rao

4. Generation of Human Induced Pluripotent Stem Cells by Lentiviral Transduction

Jennifer C. Moore

5. Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus

Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri, and Chad C. MacArthur

6. Generation of Human Induced Pluripotent Stem Cells (hiPSCs) using Episomal Vectors on Defined Essential 8TM Medium Conditions

Andrew Fontes, Chad C. MacArthur, Pauline T. Lieu, and Mohan C. Vemuri

7. Feeder-free Substrates for Pluripotent Stem Cell Culture

Alexandria Sams and Mark J. Powers

Part II. Culture and Differentiation of Pluripotent Stem Cells

8. Methods for Culturing Human Embryonic Stem Cells on Feeders

Jasmeet Kaur and Mary Lynn Tilkins

9. Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System

Jasmeet Kaur, Mary Lynn Tilkins, and Shayne Boucher

10. Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage

Dennis Van Hoof and Muluye E. Liku

11. Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes

Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie

12. Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocytes

Paul W. Burridge and Elias T. Zambidis

13. Generation of iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal Vectors

Kejin Hu and Igor Slukvin

Part III. Pluripotent Stem Cell Characterization

14. Cellular Characterization of Human Pluripotent Stem Cells

Rene H. Quintanilla Jr.

15. TaqMan® OpenArray® High Throughput Transcriptional Analysis of Human Embryonic and Induced Pluripotent Stem Cells

Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancebo, Janice Au-Young, and Elena Grigorenko

16. Epigenome Analysis of Pluripotent Stem Cells

Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart

17. Single Cell Gene Expression Analysis of Pluripotent Stem Cells

Ronald V. Abruzzese and Richard A. Fekete

18. Profiling Stem Cells Using Quantitative PCR Protein Assays

David Ruff and Pauline Lieu

Part IV. Pluripotent Stem Cell Modification and Applications

19. Labeled Stem Cells as Disease Models and in Drug Discovery

Catharina Ellerström, Raimund Strehl, and Johan Hyllner

20. Cloning Technologies

Andrew Fontes

21. Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells

Yin Liu, Kate Wagner, and Uma Lakshmipathy

22. Site-Specific Integration in Human ESC Using Jump-In(TM) TI(TM) Technology

Chad C. MacArthur