Plant Cell Morphogenesis: Methods and Protocols

1. Essential Methods of Plant Sample Preparation for Light Microscopy

Ales Soukup and Edita Tylová

2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

Ales Soukup

3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues

Kieran J. D. Lee and J. Paul Knox

4. Automated Microscopy in Forward Genetic Screening of Arabidopsis

Tereza Dobisová and Jan Hejátko

5. Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections

Jana Albrechtová, Zuzana Kubínová, Ales Soukup, and Jirí Janácek

6. Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells

Andreas Nebenführ

7. Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM

Amparo Rosero, Viktor Zárský, and Fatima Cvrcková

8. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces

Dorota Kwiatkowska and Agata Burian

9. Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope

Olivier Hamant, Pradeep Das, and Agata Burian

10. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX

Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith

11. Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy

Anne-Lise Routier-Kierzkowska and Richard S. Smith

12. High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy

Ichirou Karahara and Byung-Ho Kang

13. Reconstructing Plant Cells in 3D by Serial Section Electron Tomography

Kiminori Toyooka and Byung-Ho Kang

14. Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy

Jindriska Fiserová and Martin W. Goldberg

15. Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections

Ilse Foissner and Margit Hoeftberger

16. Live Cell Imaging of Arabidopsis Root Hairs

Tijs Ketelaar

17. Morphological Analysis of Cell Growth Mutants in Physcomitrella

Luis Vidali and Jeffrey P. Bibeau

18. Plant Cell Lines in Cell Morphogenesis Research

Daniela Seifertová, Petr Klíma, Markéta Parezová, Jan Petrásek, Eva Zazímalová, and Zdenek Opatrný

19. Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes

Radek Bezvoda, Roman Pleskot, Viktor Zárský, and Martin Potocký

20. Lab-on-a-Chip for Studying Growing Pollen Tubes

Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann

21. Laser Microdissection of Plant Cells

Yvonne Ludwig and Frank Hochholdinger

22. Optical Trapping in Plant Cells

Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren

23. Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery

Fatima Cvrcková and Michal Hála