Macrophages and Dendritic Cells: Methods and Protocols

1. Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors Georgia Schäfer and Gordon D. Brown2. Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite InteractionsAlessandra Mortellaro, Matteo Urbano, Stefania Citterio, Maria Foti, Francesca Granucci, and Paola Ricciardi-Castagnoli3. Macrophage Activation: Classical Versus AlternativeAndrea Classen, Jorge Lloberas, and Antonio Celada4. Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence MicroscopyBenjamin E. Steinberg and Sergio Grinstein5. Detection of Activated Rab7 GTPase with an Immobilized RILP ProbeJim Sun, Ala-Eddine Deghmane, Cecilia Bucci, and Zakaria Hmama6. Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and ReconstitutionChen Varol, Limor Landsman, and Steffen Jung7. Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface ReceptorsHsi-Hsien Lin, Gin-Wen Chang, Yi-Shu Huang, Cheng-Chih Hsiao, Martin Stacey, and Siamon Gordon8. Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose ReceptorLuisa Martinez-Pomares9. The Expression of Exogenous Genes in Macrophages: Obstacles and OpportunitiesXia Zhang, Justin P. Edwards, and David M. Mosser10. Genetic Modification of Murine Dendritic Cells by RNA TransfectionMaurizio Ceppi, Enrico Schmidt, and Philippe Pierre11. Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities Robin M. Yates, Albin Hermetter, and David G.Russell12. Derivation and Characterization of Murine Alternatively Activated (M2) MacrophagesVictor W.H. Ho and Laura M. Sly13. Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell BiologyMattias Svensson14. Generation, Culture and Flow-Cytometric Characterization of Primary Mouse MacrophagesUlrike Schleicher and Christian Bogdan15. Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient MiceFrank Brombacher, Berenice Arendse, Reagon Peterson, Alexandra Hölscher, and Christoph Hölscher16. Analysis of Matrix Metalloproteinase Secretion by MacrophagesPaul T. Elkington, Justin A. Green, and Jon S. Friedland17. Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug TargetsLot de Witte, Marein A.W.P. de Jong, Jeroen den Dunnen, Yvette van Kooyk, and Teunis B.H. Geijtenbeek18. Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines Jimmy S. Lee and Neil E. Reiner19. Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages Vivian Kwok, Eric Vachon, and Gregory P. Downey20. Large Scale Phagosome PreparationAdrien F. Vinet and Albert Descoteaux21. Coordinate Regulation of Sphingosine Kinase and Actin DynamicsShankar S. Iyer and David J. Kusner