Plant Biotechnology and Medicinal Plants: Periwinkle, Milk Thistle and Foxglove

Chapter 1: Terpenoid indole alkaloid production from in vitro culture of Catharanthus roseus through biotic, abiotic elicitation and precursors feeding.
1-Abiotic elicitation and TIA production from in vitro cultures of C. roseus 1-1-Media composition and pH 1-1-1-Media components 1-1-2-Medium pH
1-2- Growth regulators and phytohormones 1-2-1-Auxins and cytokinins 1-2-2-Methyl jasmonate
1-3-Light and ultraviolet (UV)-B light 1-3-1-Photoperiod regime 1-3-2-UV-B light
1-4-Temperature and deprivation of oxygen 1-4-1- Temperature 1-4-2-Deprivation of oxygen
1-5- Biomass density and subculture cycles 1-5-1-Inoculum size 1-5-2-Subculture cycles
1-6-Bioregulators
1-7-Permeabilizing agent and chemicals
1-8-Long-term preservation
1-9-Small and high volume scale up
Chapter 2: Biotic elicitation and TIA production from in vitro cultures of C.roseus
Chapter 3: Precursor feeding and TIA production from in vitro cultures of C.roseus
Chapter 4: Conclusion and future aspects
Sections on S. marianum and Digitalis will follow the same structural organization as described above for C. roseus