Biolistic DNA Delivery in Plants: Methods and Protocols

Part I: Tribute to Professor Diter Von Wettstein

1. Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications

Sachin Rustgi and Birgitte Skadhauge

2. Diter von Wettstein and The Meiotic Program of Pairing and Recombination

Denise Zickler

Part II: Background and Overview

3. A Short History and Perspectives on Plant Genetic Transformation

Thakku R. Ramkumar, Sangram K. Lenka, Sagar S. Arya, and Kailash C. Bansal

4. Choice of The Promoter for Tissue and Developmental Stage-specific Gene

Expression

Olga G. Smirnova and Alex V. Kochetov

5. Choice of Explant for Plant Genetic Transformation

Nibedita Chakraborty, Priyanka Chakraborty, Moutushi Sen, and Rajib Bandopadhyay

6. Biolistic Approach for Transient Gene Expression Studies in Plants

Benoît Lacroix, Vitaly Citovsky

7. Nano-biolistics: An Emerging Genetic Transformation Approach

Part II: Protocols

8. Biolistic Transformation of Japonica Rice Varieties

Raviraj Banakar and Kan Wang

Kan Wang, Huilan Zhu, and Morgan McCaw

10. Biolistic DNA Delivery and its Applications in Sorghum bicolor

Guoquan Liu, Karen Massel, Basam Tabet, and Ian D. Godwin

11. Biolistic-mediated Gene Delivery in Sugarcane

Priya A. Joyce and Yue Sun

12. Genetic Transformation of Common Wheat (Triticum aestivum L.) Using Biolistics

Caroline A. Sparks, Angela Doherty

13. Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds

Man Zhou, Junming Zhao, Dayong Li, Shuangrong Yuan, Ning Yuan, Zhigang Li, Haiyan Jia, Fangyuan Gao, Bekir San, Qian Hu, and Hong Luo

14. Use of Microspore-derived Calli as Explants for Biolistic Transformation of Common Wheat

Sachin Rustgi, Samneet Kashyap, Nii Ankrah, and Diter von Wettstein

15. Plant Transformation Techniques: Agrobacterium- and Microparticle-mediated Gene Transfer in Cereal Plants

Jafargholi Imani and Karl-Heinz Kogel

16. Proteolistics: A Protein Delivery Method

Susana Martin-Ortigosa and Kan Wang

17. Biolistic Delivery of Programmable Nuclease (CRISPR/Cas9) in Bread Wheat

Abhishek Bhandawat, Vinita Sharma, Vikas Rishi, and Joy Roy