Plant Endosomes: Methods and Protocols

1. Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis

Akira Yoshinari and Junpei Takano

2. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles

Mengqian Luo, Ying Zhu, Zhiqi Liu, and Liwen Jiang

3. Analysis of Endoplasmic Reticulum-Endosomes Association Using Live-Cell Imaging In Plant Cells

Giovanni Stefano and Federica Brandizzi

4. Degradation of Abscisic Acid Receptors through the Endosomal Pathway

Borja Belda-Palazón and Pedro L. Rodriguez

5. Biochemical and Imaging Analysis of ALIX Function in the Endosomal Trafficking of Arabidopsis Protein Cargoes

Marta García-León and Vicente Rubio

6. Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network

Pengfei Wang and Byung-Ho Kang

7. Imaging Plant Cells by High-pressure Freezing and Serial Block-Face Scanning Electron Microscopy

Kirk Czymmek, Abhilash Sawant, Kaija Goodman, Janice Pennington, Pal Pedersen, Mrinalini Hoon, and Marisa S. Otegui

8. Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking

10. Subcellular Localization of PI3P in Arabidopsis

Han Nim Lee, Hyera Jung, and Taijoon Chung

12. Cell-free Protein Translation System For Expression Of Lipid-binding Proteins Tagged with Small Epitopes and their use in Protein-lipid Overlay Assays

13. Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants

Guangxi Ren, Michel Ruiz Rosquete, Angelo G. Peralta,  Sivakumar Pattathil, Michael G. Hahn, Thomas Wilkop, and Georgia Drakakaki

Karin Vogel and Erika Isono

15. Purification and Interaction Analysis of a Plant-specific RAB5 Effector by in vitro pull-down Assay 

Emi Ito, Seung-won Choi, and Takashi Ueda