Molecular Neurobiology: Recombinant DNA Approaches

'1. The Molecular Biology of the Na,K-ATPase and Other Genes Involved in the Ouabain-Resistant Phenotype.- 1. Introduction.- 2. Molecular Cloning of the Na,K-ATPase Catalytic Subunit.- 3. Organization and Expression of the Rat Sodium Pump a-Subunit Gene.- 4. Organization and Expression of the a-Subunit Gene in Ouabain-Resistant Cell Lines.- 5. Isolation and Characterization of a Ouabain-Resistance Gene.- 6. Transfer of the Sodium Pump a-Subunit Gene Confers Ouabain Resistance to Ouabain-Sensitive Cells.- 7. Isolation of Genes Related to the Sodium Pump and Their Expression in a Ouabain-Resistant Cell Line.- 8. Conclusions and Future Prospects.- References.- 2. Molecular Biology of the Genes Encoding the Major Myelin Proteins.- 1. Introduction.- 2. Formation and Structure of the Myelin Sheath.- 3. Myelin-Specific Proteins.- 3.1. Myelin Basic Protein.- 3.2. Po.- 3.3. Proteolipid Protein.- 3.4. Other Myelin Proteins.- 4. Conclusion.- References.- 3. Molecular Biology of the Neural and Muscle Nicotinic Acetylcholine Receptors.- 1. Introduction.- 2. Isolation of cDNA Clones Coding for the Acetylcholine Receptor Expressed in the Torpedo Electric Organ.- 2.1. Torpedo ?-Subunit.- 2.2. Torpedo ?-Subunit.- 2.3. Torpedo Clone 3C1.- 3. Isolation of cDNA Clones Coding for Mouse Skeletal Muscle Acetylcholine Receptor.- 3.1. Mouse ?-Subunit.- 3.2. Mouse ?-Subunit.- 3.3. Mouse ?-Subunit.- 3.4. Mouse ?-Subunit.- 3.5. Expression of Mouse Receptor in Oocytes.- 4. Structure of the Acetylcholine Receptor.- 4.1. Primary Structure.- 4.2. Folding through the Membrane.- 4.3. Acetylcholine-Binding Site.- 5. Expression of the Acetylcholine Receptor Genes in Skeletal Muscle.- 5.1. Skeletal Muscle Denervation.- 5.2. Synaptic versus Extrasynaptic Acetylcholine Receptor.- 6. Brain Receptors.- 6.1. Neuronal a-Subunit Clone.- 6.2. Structure of the Neuronal ?-Subunit.- 6.3. Expression of the Neural ?-Subunit RNA.- 6.4. Evidence for a Gene Family.- 7. Conclusion.- References.- 4. Molecular Biology of Muscle Development: The Myosin Gene Family of Caenorhabditis elegans.- 1. Introduction.- 2. Genetics of the unc-54 Locus.- 2.1. Selection of unc-54 Mutations.- 2.2. Deletions and Duplications of the unc-54 Locus.- 2.3. Orientation of the unc-54 Gene.- 2.4. In Situ Hybridization to Chromosomes.- 2.5. Interactions with Other Genes.- 3. Immunological Identification and Localization of Myosin Isoforms.- 3.1. Production of Four Sarcomeric Myosin Heavy-Chain Isoforms by Caenorhabditis elegans.- 3.2. Monoclonal Antibodies Specific for Different Myosin Isoforms.- 3.3. Two Myosins Required to Make the Body Wall Thick Filament.- 4. Molecular Cloning of the unc-54 and myo-1,2,3 MHC Genes.- 4.1. Cell-Free Translation of MHC mDNA.- 4.2. unc-54 cDNA Clones.- 4.3. Cloning the unc-54 Gene.- 4.4. Identification of the myo-1,2,3 MHC Genes by Homology to unc-54.- 5. Immunological Identification of the Products of the myo-1,2,3 MHC Genes.- 6. Structural Organization of the Nematode MHC Genes.- 6.1. Highly Conserved Head Sequences and Variable Rod Sequences.- 6.2. Selective Loss of Introns from the Nematode MHC Genes.- 6.3. Unusual Features at Intron Junctions.- 6.4. Terminal Sequences.- 7. Molecular Anatomy of the Myosin Molecule.- 7.1. Topography of the Head.- 7.2. Rod Sequences.- 8. Sequences and Molecular Interpretation of unc-54 Mutations.- 8.1. Rapid Cloning and Sequencing of Mutations.- 8.2. Correlation of Physical and Genetic Fine-Structure Map.- 8.3. Deletions in the Rod.- 8.4. Nonsense Mutations and Suppressors.- 8.5. Mutations Affecting the Synthesis of MHC.- 8.6. A Dominant Assembly-Defective Missense Mutation.- 8.7. Mutations in the Head.- 8.8. Mechanisms of Mutagenesis in the Nematode..- 9. Myosin Protein Expression and Mutagenesis in E. coli.- References.- 5. Small Cardioactive Peptides in A and B: Chemical Messengers in the Aplysia Nervous System.- 1. Introduction.- 2. The