High-Throughput Protein Production and Purification: Methods and Protocols

Part 1 General Procedures

1)      HTP cloning

2)      HTP generation of truncation and mutation libraries

3)      HTP protein production in Escherichia coli

4)      HTP Production of Protein Complexes by Co-expression in Escherichia coli

5)      HTP protein complex reconstitution in Escherichia coli

6)      HTP protein production in alternative bacterial system

7)      HTP protein production in Yeast

8)      HTP protein production and protein complexes in Baculovirus

9)      HTP protein production and protein complexes in Mammalian cells

11)  HTP protein expression in bacterial Cell Free system

12)  HTP protein expression in eukaryotic Cell Free system

13)  HTP refolding of proteins

14)  HTP production of binders

15)  HTP production of antibodies

16)  HTP Modification of strains for improved protein expression (CHO, HEK, E. coli)

Part 2 Case studies

17)  HTP production of membrane proteins

18)  HTP membrane protein expression in bacterial Cell Free system

20)  HTP production of small cysteine-rich proteins (VENOMICS)

21)  HTP production of transcription factors

22)  HTP Protein production for drug discovery

Part 3 HTP micro-characterizations of proteins

23)  Analytical SEC- MALLS

24)  Differential Scanning Fluorimetry

25)  Native mass spectrometry

26)  Protein-protein interaction (Pull-down/Hold-up)

28)  Analytical Electron microscopy