The Initiation of DNA Replication in Eukaryotes

1. Introduction to DNA replication initiation in eukaryotes.- 2. Choice of origins and replication timing control in yeast.- 3. Replication timing and initiation in metazoans.- 4. Replication timing gradients and origin activation.- 5. Genome wide localization of replication initiation factors.- 6. Genome scale analysis of metaozoan replication origins.- 7. Role of dormant origins in replication initiation.- 8. Centromeres and DNA replication initiation.- 9. Rif1 regulation of replication timing.- 10. Role of ORC in replication initiation.- 11. Licensing of replication origins- loading Mcm2-7.- 12. Role of chromatin in replication initiation.- 13. Role of Mcm2-7 in replication initiation.- 14. Role of CDK in replication initiation.- 15. Role of DDK in replication initiation.- 16. Roles of Sld2, Sld3, and Dpb11 in replication initiation.- 17. Role of Mcm10 in replication initiation.- 18. Role of post-translational modifications in replication initiation.- 19. Assembly of the Cdc45-Mcm2-7-GINS complex, the replication helicase.- 20. Activity of the Cdc45-Mcm2-7-GINS complex, the replication helicase.- 21. Structure function studies of replication initiation factors.- 22. Pol-alpha activation and coupling with helicase unwinding.- 23. Replication initiation and DNA damage.- 24. Protein phosphatases and replication initiation.- 25. Spindle checkpoints and replication initiation.- 26. Protein degradation and replication initiation.- 27. Break-induced replication.- 27. Meier Gorlin syndrome.- 28. Replication Stress and Cancer.