Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening f

Introduction.- SECTION I. METHODOLOGY AND TECHNIQUES.- Methods and instrumentation in mass spectrometry for the differentiation of closely related microorganisms.- Sample preparation methods for the rapid MS analysis of microorganisms.- Advantages offered by proteomic strategies for rapid biodetection.- Bottom Up Proteomics Methods for Strain-Level Typing and Identification of Bacteria.- Maximizing the taxonomic resolution of MALDI-TOF-MS-based approaches to bacterial characterization: From culture conditions through data analysis.- SECTION II. SUB-SPECIES DISCRIMINATION.-

Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains.- Discriminatory Power of MALDI-TOF Mass Spectrometry for Phylogenetically Closely Related Microbial Strains.- MALDI-TOF MS as a novel tool for dereplication and characterization of microbiota in bacterial diversity studies.- Bacterial Identification at the Serovar level by Top-down Mass Spectrometry.- SECTION III. DRUG RESISTANCE MONITORING AND ASSAYS.- Rapid profiling of human pathogenic bacteria and antibiotic resistance employing specific tryptic peptides as biomarkers.- Detection of beta-lactamases and their activity using MALDI-TOF MS.- Stable-isotope based strategies for rapid determination of drug resistance by mass spectrometry.