Advanced Technologies for Protein Complex Production and Characterization

Preface. Complex Biologics: Engineering the Tools and the Hosts. M. Cristina Vega, Francisco J. Fernández and Imre Berger

Section 1: Introduction

1. Protein complex production from the drug discovery standpoint; Ismail Moarefi     

<2. Choose a suitable expression host: a survey of available protein production platforms; Francisco J. Fernández and M. Cristina Vega

Section 2: Prokaryotic expression hosts

3. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts; Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel and Imre Berger

4. Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput; Renaud Vincentelli and Christoph Romier

5. Membrane protein production in E. coli for applications in drug discovery; Harm Jan (Arjan) Snijder and Jonna Hakulinen

6. Cell-free Synthesis of Macromolecular Complexes; Matthieu Botte, Aurélien Deniaud and Christiane Schaffitzel                                                                                         

7. A Bacillus megaterium system for the production of recombinant proteins and protein complexes; Rebekka Biedendieck

8. Protein complex production in alternative prokaryotic hosts; Sara Gómez, Miguel López-Estepa, Francisco J. Fernández and M. Cristina Vega                              

Section 3: Lower Eukaryotic expression hosts

9. Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts; Francisco J. Fernandez, Miguel López-Estepa, Javier Querol-García and M. Cristina Vega

10. Leishmania tarentolae for the Production of Multi-Subunit Complexes; Tomoaki Niimi

11. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes; Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez and M. Cristina Vega

Section 4: Higher Eukaryotic expression hosts

12. Fundamentals of baculovirus expression and applications; Tom A. Kost and Christopher W. Kemp

13. The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics; Duygu Sari, Kapil Gupta, Deepak Balaji Govinda Raj, Alice Aubert, Petra Dmcova, Frederic Garzoni, Daniel Fitzgerald and Imre Berger  

14. Fundamentals of expression in mammalian cells; Michael R. Dyson 

Section 5: Plant expression

16. Microalgae as solar-powered protein factories; Franziska Hempel and Uwe G. Maier

17. Strategies and methodologies for the co-expression of multiple proteins in plants; Albert Ferrer, Montserrat Arró, David Manzano and Teresa Altabella                

18. Transient expression systems in plants-potentialities and constraints; Tomas Canto         

19. Complex reconstitution from individual protein modules; Jérome Basquin, Michael Taschner and Esben Lorentzen                                                                             

20. Structural reconstruction of protein-protein complexes involved in intracellular signaling; Klára Kirsch, Péter Sok, and Attila Remény                                                           

Section 7: Biophysical methods to assess protein complexes

21. The use of small-angle scattering for the characterization of multi subunit complexes; Adam Round

22. Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. Filippo Prischi and Annalisa Pastore