NLR Proteins: Methods and Protocols
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- Artikel-Nr.: 10505472
Beschreibung
Innate Immune Receptors.- Atypical Inflammasomes.- Assessment of Inflammasome Activation By Cytokine And Danger Signal Detection.- Investigating IL-1beta Secretion Using Real-Time Single-Cell Imaging.- Measuring IL-1beta Processing By Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor.- Measuring IL-1beta Processing By Bioluminescence Sensors II: The Igluc System.- Assessing Extracellular ATP as Danger Signal In Vivo: The Pmeluc System.- Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-Immunoprecipitation and Cross-Linking.- Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence.- Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer.- Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity Of Inflammasome Components.- Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay.- Assessing Caspase-1 Activation.- Cell-Free Assay for Inflammasome Activation.- Functional Reconstruction of Nlrs In HEK293 Cells.- Method to Measure Ubiquitinization of Nlrs.- Cytofluorometric Quantification of Cell Death Elicited By NLR Proteins.- NLR in Human Diseases: Role and Laboratory Findings.
Eigenschaften
Breite: | 181 |
Gewicht: | 710 g |
Höhe: | 262 |
Länge: | 21 |
Seiten: | 256 |
Sprachen: | Englisch |
Autor: | Francesco Di Virgilio, Pablo Pelegrín |
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