Biomimetics and Stem Cells: Methods and Protocols

1. Derivation and Network Formation of Vascular Cells from Human Pluripotent Stem Cells

Sravanti Kusuma, Bria Macklin, and Sharon Gerecht

2. High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis

Ahmad E. Abu-Hakmeh and Leo Q. Wan

3. Microfluidic Device to Culture 3D In Vitro Human Capillary Networks

Monica L. Moya, Luis F. Alonzo, and Steven C. George

4. Multi-Function Co-Culture Model for Evaluating Cell-Cell Interactions

Danielle R. Bogdanowicz and Helen H. Lu

5. Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography

Camila Londono, John Soleas, Petra B Lücker, Suthamathy Sathananthan, J. Stewart Aitchison, and Alison P. McGuigan

6. Bioreactor Cultivation of Anatomically-Shaped Human Bone Grafts

Joshua P. Temple, Keith Yeager, Sarindr Bhumiratana, Gordana Vunjak-Novakovic, and Warren L. Grayson

7. Determining the Role of Matrix Compliance in the Differentiation of Mammary Stem Cells

KangAe Lee and Celeste M. Nelson

8. Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules

Anthony Conway, Dawn P. Spelke, and David V. Schaffer

9. An Assay to Quantify Chemotactic Properties of Degradation Products from Extracellular Matrix

Brian M. Sicari, Li Zhang, Ricardo Londono, and Stephen F. Badylak

10. Biomimetic Strategies Incorporating Enzymes into CaP Coatings Mimicking the In Vivo Environment

Ana M. Martins and Rui L. Reis

11. Fabrication of Biofunctionalized, Cell-Laden Macroporous 3D PEG Hydrogels as Bone Marrow Analogs for the Cultivation of Human Hematopoietic Stem and Progenitor Cells

Lisa Rödling, Annamarija Raic, and Cornelia Lee-Thedieck

12. Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation

Busra Mammadov, Mustafa O. Guler, and Ayse B. Tekinay

13. The Delivery and Evaluation of RNAi Therapeutics for Heterotopic Ossification Pathologies

Arun R. Shrivats and Jeffrey O. Hollinger

14. Mimicking Bone Microenvironment for Directing Adipose Tissue-Derived Mesenchymal Stem Cells into Osteogenic Differentiation

ZuFu Lu, Seyed-Iman Roohani-Esfahani, and Hala Zreiqat

15. Cultivation of Human Bone-Like Tissue from Pluripotent Stem Cell-Derived Osteogenic Progenitors in Perfusion Bioreactors

Giuseppe Maria de Peppo, Gordana Vunjak-Novakovic, and Darja Marolt