Plant Endosomes: Methods and Protocols

The Use of FM Dyes to Analyze Plant Endocytosis.- Sterol Dynamics During Endocytic Trafficking in Arabidopsis.- Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs.- Analysis of Fluid Phase Endocytosis in (Intact) Plant Cells.- Immunogold Labeling and Electron Tomography of Plant Endosomes.- Investigating Protein-Protein Interactions in the Plant Endomembrane System Using Multiphoton-Induced FRET-FLIM.- Analysis of Rab GTPase-Effector Interactions by Bimolecular Fluorescence Complementation.- In Vivo Imaging of Brassinosteroid Endocytosis in Arabidopsis.- Analysis of Prevacuolar Compartment-Mediated Vacuolar Proteins Transport.- Evaluation of Defective Endosomal Trafficking to the Vacuole by Monitoring Seed Storage Proteins in Arabidopsis thaliana.- Trans-Species Complementation Analysis to Study Function Conservation of Plant Endosomal Sorting Complex Required for Transport (ESCRT) Proteins.- A Re-Elicitation Assay to Correlate flg22-Signaling Competency with Ligand-Induced Endocytic Degradation of the FLS2 Receptor.- Preparation of Enriched Plant Clathrin-Coated Vesicles by Differential and Density Gradient Centrifugation.- Proteomics of Endosomal Compartments from Plants Case Study: Isolation of trans -Golgi Network Vesicles.- Analysis of Global Ubiquitylation and Ubiquitin-Binding Domains Involved in Endosomal Trafficking.- Analysis of Endocytosis and Ubiquitination of the BOR1 Transporter.- Ubiquitination of Plant Immune Receptors.- Ubiquitylation-Mediated Control of Polar Auxin Transport: Analysis of Arabidopsis PIN2 Auxin Transport Protein.- Chemical Genomics Screening for Biomodulators of Endomembrane System Trafficking.- Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis.