Nuclear Reprogramming: Methods and Protocols

1. Nuclear Transfer in the Mouse

Vincent Brochard and Zichuan Liu

2. Nuclear Transfer in the Rabbit

Nathalie Daniel and Patrick Chesné

3. Nuclear Transfer in Ruminants

Joon-Hee Lee and Walid E. Maalouf

4. Nuclear Transfer and Transgenesis in the Pig

Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, and Eckhard Wolf

5. Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation

Huseyin Sumer and Paul J. Verma

6. Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte

Jerome Jullien

7. Assessing the Quality of Donor Cells: Karyotyping Methods

Amélie Bonnet-Garnier, Anne-Clemence Veillard, Bertrand Bed'Hom, Hélène Hayes, and Janice Britton-Davidian

8. Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer

Eiji Mizutani, Sayaka Wakayama, and Teruhiko Wakayama

9. Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos

Claire Boulesteix and Nathalie Beaujean

10. Visualization of Epigenetic Modifications in Preimplantation Embryos

Hiroshi Kimura and Kazuo Yamagata

11. Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer

Sebastian T. Balbach and Michele Boiani

12. Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming

Olga Østrup, Hanne S. Pedersen, Hanne M. Holm, and Poul Hyttel

13. Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining

Claire Chazaud

14. Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR)

Nathalie Peynot, Véronique Duranthon, and Daulat Raheem Khan

15. Studying Bovine Early Embryo Transcriptome by Microarray

Isabelle Dufort, Claude Robert, and Marc-André Sirard

16. Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level

Annick Lefèvre and Thierry Blachère

17. Micro Chromatin Immunoprecipitation ( ChIP) from Early Mammalian Embryos

John Arne Dahl and Arne Klungland

18. Assessing Reprogramming by Chimera Formation and Tetraploid Complementation

Xin Li, Bao-long Xia, Wei Li, and Qi Zhou

19. Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology

Bernd Püschel and Alice Jouneau

20. Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)

Hélène Kiefer