Nuclear G-Protein Coupled Receptors: Methods and Protocols

1. Studies of Intracellular Angiotensin II

Richard N. Re and Julia L. Cook

2. Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes

Clémence Merlen and Jonathan Ledoux

3. Design and Application of Light-Activated Probes for Cellular Signaling

David Chatenet, Steve Bourgault, and Alain Fournier

4. Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes

Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen

5. Quantification of Catecholamine Uptake in Adult Cardiac Myocytes

Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell

6. Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase

Chantal Binda and Jean-Luc Parent

7. Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes

Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel

8. High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs)

Vikrant K. Bhosle, Fernand Gobeil Jr., Jose Carlos Rivera, Alfredo Ribeiro-da-Silva, and Sylvain Chemtob

9. Biochemical Fractionation of Membrane Receptors in the Nucleus

Ying-Nai Wang, Longfei Huo, Jennifer L. Hsu, and Mien-Chie Hung

10. Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons

Yuh-Jiin I. Jong and Karen L. O'Malley

11. Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors

Karen A. Ryall and Jeffrey J. Saucerman

12. Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]

Senka Ljuboyevic and Donald M. Bers

13. Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors

Julie Bossuyt and Donald M. Bers

14. Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting Proteins

Rhiannon Campden, Darlaine Pétrin, Mélanie Robitaille, Nicolas Audet, Sarah Gora, Stéphane Angers, and Terence E. Hébert

15. Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei

George Vaniotis, Sarah Gora, André Nantel, Terence E. Hébert, and Bruce G. Allen

16. Trafficking and Function of GPCRs in the Endosomal Compartment

Davide Calebiro, Amod Godbole, Sandra Lyga, and Martin J. Lohse