Imaging and Tracking Stem Cells: Methods and Protocols

1. Primary Culture and Live-Imaging of Adult Neural Stem Cells and Their Progeny

Felipe Ortega, Benedikt Berninger, and Marcos R. Costa

2. Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology

Marie-Therese Armentero, Patrizia Bossolasco, and Lidia Cova

3. High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes

Gábor Földes and Maxime Mioulane

4. A High Content Imaging-Based Approach for Classifying Cellular Phenotypes

Joseph J. Kim, Sebastián L. Vega, and Prabhas V. Moghe

5. Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions

Go Nagamatsu and Toshio Suda

6. Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells

Youbo Zhao, Andrew J. Bower, Benedikt W. Graf, Marni D. Boppart, and Stephen A. Boppart

7. Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells

Jian Tajbakhsh

8. Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention

HuaLei Zhang and Rong Zhou

9. Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice

Takaya Abe, Shinichi Aizawa, and Toshihiko Fujimori

10. Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo

Minjung Kang, Panagiotis Xenopoulos, Silvia Muñoz-Descalzo, Xinghua Lou, and Anna-Katerina Hadjantonakis

11. Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Post-Mortem Multi-Color Histological Analysis

Kristien Reekmans, Nathalie De Vocht, Jelle Praet, Debbie Le Blon, Chloé Hoornaert, Jasmijn Daans, Annemie Van der Linden, Zwi Berneman, and Peter Ponsaerts

12. Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells

Andrea Farini, ChiaraVilla, Marzia Belicchi, Mirella Meregalli, and Yvan Torrente

13. Non-Invasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging

Annette Tennstaedt, Markus Aswendt, Joanna Adamczak, and Mathias Hoehn

14. Magnetic Resonance Imaging and Tracking of Stem Cells

Hossein Nejadnik, Rostislav Castillo, and Heike E. Daldrup-Link

15. Whole Body MRI Followed by Fluorescent Microscopy Analysis for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intracardiac Injection, Skeletal Muscle Transplantation, or Systemic Delivery

Boris Odintsov, Ju Lan Chun, and Suzanne E. Berry

16. Molecular Imaging and Tracking Stem Cells in Neurosciences

Toma Spiriev, Nora Sandu, and Bernhard Schaller

17. Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction

Weijun Su, Liang Leng, Zhongchao Han, Zuoxiang He, and Zongjin Li