Stem Cell Nanotechnology: Methods and Protocols

1. Propagation and Differentiation of Human Wharton's Jelly Stem Cells on Three Dimensional Nanofibrous Scaffolds

Kalamegam Gauthaman, Chui-Yee Fong, Jayarama Reddy Venugopal, Arijit Biswas, Seeram Ramakrishna, and Ariff Bongso

2. Composite Electrospun Nanofibers for Influencing Stem Cell Fate

Alessandro Polini, Silvia Scaglione, Rodolfo Quarto, and Dario Pisignano

3. In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells

Rosario Sanchez-Martin, Victoria Cano-Cortés, Juan Antonio Marchal, and Macarena Perán

4. Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells

Luisa Campagnolo, Ivana Fenoglio, Micol Massimiani, Andrea Magrini, and Antonio Pietroiusti

5. Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing

Seher Ustun, Samet Kocabey, Mustafa O. Guler, and Ayse B. Tekinay

6. Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiation of Mesenchymal Stem Cells

Cristian Pablo Pennisi, Vladimir Zachar, Trine Fink, Leonid Gurevich, and Peter Fojan

7. Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer

Sarah Rieck, Katrin Zimmermann, and Daniela Wenzel

8. Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-Cultures

Sounderya Nagarajan and Yong Zhang

9. Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers

Marco A. Alvarez-Perez, Vincenzo Guarino, Valentina Cirillo, and Luigi Ambrosio

10. General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds

A. Géraldine Guex, Giuseppino Fortunato, Dirk Hegemann, Hendrik T. Tevaearai, and Marie-Noëlle Giraud

11. Mesenchymal Stem Cells and Nano-Structured Surfaces

Yinghong Zhou, Nishant Chakravorty, Yin Xiao, and Wenyi Gu

12. Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds

Somaieh Kazemnejad, Amir Hasan Zarnani, Manijeh Khanmohammadi, and Sahba Mobini

13. Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Encapsulated Neural Stem Cells

Carla Cunha, Silvia Panseri, and Fabrizio Gelain