Plant Cytogenetics: Methods and Protocols

1. C -Banding of Plant Chromosomes
Eric N. Jellen

2. Chromosome Painting by GISH and Multi-Color
FISH
Steven S. Xu, Zhao Liu, Qijun Zhang,
Zhixia Niu, Chao-Chien Jan, and Xiwen Cai

3. Fluorescent In Situ Hybridization on Extended Chromatin Fibers for
High-Resolution Analysis of Plant Chromosomes
Daryna Dechyeva and Thomas Schmidt

4. Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying
Homologous Chromosomes
Araceli Fominaya, Yolanda Loarce,
Juan M. González, and Esther Ferrer

5. Localization
of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Miroslava Karafiátová, Jan Bartos,
Jaroslav Dolezel

6. Immunolabelling
and In Situ Labelling of Isolated
Plant Interphase Nuclei
Ali Pendle and Peter Shaw

7. Manipulation
of Homologous Chromosome Recombination in Wheat
Adam J. Lukaszewski

8. Dissecting
Plant Chromosomes by the Use of Ionizing Radiation
Penny M.A. Kianian, Katie L.
Liberatore, Marisa Miller, Justin B. Hegstad, and Shahryar F. Kianian

9. Optical
Nano-Mapping and Analysis of Plant Genomes
Ming-Cheng Luo, Karin R. Deal, Armond
Murray, Tingting Zhu, Alex R. Hastie, Will Stedman, Henry Sadowski, and Michael
Saghbini

10. Flow
Sorting Plant Chromosomes
Jan Vrána, Petr Cápal,
Jarmila Cíhalíková, Marie Kubaláková, and Jaroslav Dolezel

11. Construction
of BAC Libraries from Flow-Sorted Chromosomes
Jan Safár, Hana Simková, and Jaroslav Dolezel

12. T he
Chromosome Microdissection and Microcloning Technique
Ying-Xin Zhang, Chuan-Liang Deng, and
Zan-Min Hu

13. Immunolocalization
on Whole Anther Chromosome Spreads for Male Meiosis
Stefanie Dukowic-Schulze, Anthony
Harris, and Changbin Chen

14. Mapping
Recombination Initiation Sites Using Chromatin Immunoprecipitation
Yan He, Minghui Wang, Qi Sun, and
Wojciech P. Pawlowski

15. Chromatin
Immunoprecipitation to Study Plant Epigenome
Zidian Xie and Gernot Presting