Wnt Signaling: Methods and Protocols

1. Visualizing Wnt Palmiteoylation in Single Cells

Xinxin Gao and Rami N. Hannoush

2. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction

Rubina Tuladhar, Nageswari Yarravarapu, and Lawrence Lum

3. Biochemical Methods to Analyze Wnt Protein Secretion

Kathrin Glaeser, Michael Boutros, and Julia Christina Gross

4. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum

Xinjun Zhang and Xi He

5. Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway

Xiaomo Jiang and Feng Cong

6. A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells

Annette S. Flozak, Anna P. Lam, and Cara J. Gottardi

7. Wnt-Dependent Control of Cell Polarity in Cultured Cells

Kristin B. Runkle and Eric S. Witze

8. The Use of Chick Embryos to Study Wnt Signaling/Gradients
Lisa M. Galli, Tiffany Barnes, and Laura W. Burrus

9. Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors

Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, and Natascia Tiso

10. Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo

Jesung Moon and James F. Amatruda

11. Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts

Annastasia Simone Hyde, Brian I. Hang, and Ethan Lee

12. Delivery of the Porcupine Inhibitor WNT974 in Mice

Li-shu Zhang and Lawrence Lum

13. Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis

Zhendong A. Zhong, Nicole J. Ethen, and Bart O. Williams

14. Monitoring Wnt/b-Catenin Signaling in Skin

Amy Ku, Qi Miao, and Hoang Nguyen

15. The Generation of Organoids for Studying Wnt Signaling
Jarno Drost, Benedetta Artegiani, and Hans Clevers

16. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells

Ian Huggins, David Brafman, and Karl Willert

17. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions

Xiaoping Bao, Xiaojun Lian, and Sean P. Palecek