Wheat Rust Diseases: Methods and Protocols

Preface...
Table of Contents...
Contributors...

Part I Wheat Rust Surveillance and Genotyping

1. Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping
Sajid Ali and David Hodson

2. Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance
Vanessa Bueno-Sancho, Daniel C.E. Bunting, Luis J. Yanes, Kentaro Yoshida, and Diane G.O. Saunders

3. Race Typing of Puccinia striiformis on Wheat
Mogens S. Hovmøller, Julian Rodriguez-Algaba, Tine Thach, and Chris K. Sørensen

4. Assessment of Aggressiveness of Puccinina striiformis on Wheat
Chris K. Sørensen, Tine Thach, and Mogens S. Hovmøller

5. Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing
Benjamin Schwessinger and John P. Rathjen

6. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis
Sajid Ali, Muhammad R. Khan, Angelique Gautier, Zahoor A. Swati, and Stephanie Walter

Part II Molecular Pathogenicity

7. Computational Methods for Predicting Effectors in Rust Pathogens
Jana Sperschneider, Peter N, Dodds, Jennifer M. Taylor, and Sébastien Duplessis

8. Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana
Benjamin Petre, Joe Win, Frank L.H. Menke, and Sophien Kamoun

9. Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction
Semra Hasançebi

10. Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing
Vinay Panwar and Guus Bakkeren

11. Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi
Veronica Roman-Reyna and John P. Rathjen

Part III Genetics of Rust Resistance

12. Genetic Analysis of Resistance to Wheat Rusts
Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, and Ravi P. Singh

13. Advances in Identification and Mapping of Rust Resistance Genes in Wheat
Urmil Bansal and Harbans Bariana

14. Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives
Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, and W.J. Raupp

15. Application of Genomic Selection in Breeding Wheat for Rust Resistance
Leonardo Ornella, Juan Manuel González-Camacho, Susanne Dreisigacker, and Jose Crossa

16. Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions
Adnan Riaz and Lee Hickey

Part IV Rust Resistance Gene Isolation and Characterization

17. Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning
Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, and Evans Lagudah

18. Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
Guotai Yu, Asyraf Hatta, Sambasivam Periyannan, Evans Lagudah, and Brande B.H. Wulff

19. MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
Burkhard Steuernagel, Kamil Witek, Jonathan D.G. Jones, and Brande B.H. Wulff

20. Rapid Gene Isolation using MutChromSeq
Burkhard Steuernagel, Jan Vrána, Miroslava Karafiátova, Brande B.H. Wulff, and Jaroslav Dolezel

21. Rapid Identification of Rust Resistance Genes through Cultivar-Specific de novo Chromosome Assemblies
Anupriya K. Thind, Thomas Wicker, and Simon G. Krattinger

22. BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
Li Huang

23. Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene which Encodes a Hexose Transporter
Ricky J. Milne, Katherine E. Dibley, and Evans S. Lagudah

Part V Biocontrol Agents for Rust Management

24. Biocontrol Agents for Controlling Wheat Rust
Siliang Huang and Fahu Pang