Phytochemical Properties and Antibacterial Effect Of Newbouldia laevis: Leaf Extract on Vancomycin a

Antibacterial effect of extracts of Newbouldia laevis{Tree of life} leaf on vancomycin and methicilin resistant bacteria isolates obtained from urine samples from Federal Medical Center,Owo was assayed. The leaves of N.laevis was collected from Ikole Ekiti and authenticated at the Department of Crop, Soil and Pest Management, Federal Univerrsity of Technology, Akure.The bacterial isolated from the samples namely: E.coli, K.pnuemoniae, P.aeruginosa, S.aureus and P.mirabilis were subjected to antibiotic sensitivity tests using disc diffusion method. Methanol, Chloroform, Cold and Hot water extracts of N. leavis was obtained using the cold extraction method. Phytochemical screening of the extracts were assayed and inhibitory activity of the extracts against the isolated bacteria was carried out using agar well diffusion technique. The concentration of 500mg/ml was used and the zones of inhibition recorded. The isolates were resistant to vancomycin and methicilin. The extracts revealed the presence of flavonoids, tannins, terpenes, alkanoids, phenolics, saponin and cardiac glycosides.