Bio-Carrier Vectors: Methods and Protocols

Part I: Bacterial Vectors

1. Improving E. coli Bactofection by Expression of Bacteriophage X174 Gene E

            Dongwon Park, Andrew Hill, and Blaine A. Pfeifer

2. Improved DNA Delivery Efficiency of Bacterial Vectors by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents

            Andrew N. Osahor and Kumaran Narayanan

3. Visualization of Bacteria-Mediated Gene Delivery Using High Resolution Electron and Confocal Microscopy

            Andrew N. Osahor, Allan Wee Ren Ng, and Kumaran Narayanan

Part II: Nanoparticles

4. Assessing Nucleic Acid:Cationic Nanoparticle Interaction for Gene Delivery

            Moganavelli Singh

5. Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability and Efficacy of Chemotherapeutic Drugs

            Xuemei Xie, Dan He, Yan Wu, Tingting Wang, Cailing Zhong, and Jingqing Zhang

6. Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy

            Malgorzata Grabowska, Bartosz F. Grzeskowiak, Katarzyna Rolle, and Radoslaw Mrówczynski

Part III: Peptide Vehicles

7. Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells through Protein Transduction of Transcription Factors

            Taku Kaitsuka and Kazuhito Tomizawa

8. Cardiac Targeting Peptide: From Identification to Validation to Mechanism of Transduction

            Kyle S. Feldman, Maria P. Pavlou, and Maliha Zahid

9. Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery of DNA into Cells

            Natalie J. Holl, Moumita Dey, Yue-Wern Huang, Shiow-Her Chiou, and Han-Jung Lee

10. Delivery of Peptide Nucleic Acids Using an Argininocalix[4]arene as Vector

            Alessia Finotti, Jessica Gasparello, Alessandro Casnati, Roberto Corradini, Roberto Gambari, and Francesco Sansone

Part IV: Hybrid Carriers and Other Bio Agents

11. Cell-Derived Nanovesicles as Exosome-Mimetics for Drug Delivery Purposes: Uses and Recommendations

            Yi-Hsuan Ou, Shui Zou, Wei Jiang Goh, Jiong-Wei Wang, Matthias Wacker, Bertrand Czarny, and Giorgia Pastorin

12. Hydrogel Beads of Natural Polymers as a Potential Vehicle for Colon-Targeted Drug Delivery

            Janarthanan Pushpamalar, Thenapakiam Sathasivam, and Michelle Claire Gugler

13. Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editing

            Wai Hon Chooi, Jiah Shin Chin, and Sing Yian Chew

14. Correction of Hemoglobin E/Beta-Thalassemia Patient-Derived iPSCs Using CRISPR/Cas9

            Methichit Wattanapanitch

15. CRISPR/Cas9-Mediated GFP Reporter Knock-In in K562 and Raji Cell Lines for Tracking Immune Cell Killing Assay

            Nontaphat Thongsin and Methichit Wattanapanitch

Part V: Analyzing and Profiling Gene Expression

16. Shotgun Proteomics and Mass Spectrometry as a Tool for Protein Identification and Profiling of Bio-Carrier-Based Therapeutics on Human Cancer Cells

            Syafiq Asnawi Zainal Abidin, Iekhsan Othman, and Rakesh Naidu