Pulse Field Gel Electrophoresis: Methods and Protocols

Part I: Introduction to PFGE

1. Pulsed-field Gel Electrophoresis for Disease Monitoring and Control

John Besser

2. Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network

Benjamin Félix, Sophie Roussel, and Bruno Pot

3. PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies

Marion Dalmasso and Kieran Jordan

4. Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak

Niall De Lappe and Martin Cormican

Part II: PFGE and Pathogenic Bacteria

5. Pulsed-Field Gel Electrophoresis for Listeria monocytogenes

Laura Luque-Sastre, Séamus Fanning, and Edward M. Fox

6. Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species

Qiongqiong Yan and Séamus Fanning

7. Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains

Paul Drean and Edward M. Fox

8. Pulsed-Field Gel Electrophoresis of Staphylococcus aureus

George R. Golding, Jennifer Campbell, Dave Spreitzer, and Linda Chui

9. The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile

Wondwossen A. Gebreyes and Pamela R. Fry

10. Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis

Carolina Lúquez, Lavin A. Joseph, and Susan E. Maslanka

11. Pulsed Field Gel Electrophoresis of Yersinia pestis

Tamara Revazishvili and Judith A. Johnson

12. Pulsed Field Gel Electrophoresis of Group A streptococci

Luca Agostino Vitali, Giovanni Gherardi, and Dezemona Petrelli

13. Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni

Ping Zhou and Omar A. Oyarzabal

14. Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa

Honghua Hu and Jim Manos

15. PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC

Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, and Efrain M. Ribot

16. Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica

Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, and Nicola Pugliese

PART III: PFGE and Non-Pathogenic Bacteria

17. PFGE Protocols to Distinguish Subspecies of Lactococcus lactis

Pascal Le Bourgeois, Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, and Paul Ritzenthaler

18. The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus

Tomasz Gosiewski and Monika Brzychczy-Wloch

19. Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides

Victoria Chuat and Marion Dalmasso

20. Pulsed Field Gel Electrophoresis for Bifidobacterium

Esther Jiménez, Marta Gómez, and Laura Moles

21. Pulsed Field Gel Electrophoresis for Dairy Propionibacteria

Victoria Chuat, Rosangela de Freitas, and Marion Dalmasso