Targeting Enzymes for Pharmaceutical Development: Methods and Protocols

1. In Silico Laboratory: Tools for Similarity-Based Drug Discovery

            Samo Lesnik and Janez Konc

2. The In Silico Fischer Lock-and-Key Model: The Combined Use of Molecular Descriptors and Docking Poses for the Repurposing of Old Drugs

            Marco Tutone and Anna Maria Almerico

3. Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor

4. Applications of Differential Scanning Fluorometry and Related Technologies in Characterization of Protein-Ligand Interactions

            Bolormaa Baljinnyam, Michael Ronzetti, Adam Yasgar, and Anton Simeonov

5. High-Throughput Differential Scanning Fluorimetry of GFP-Tagged Proteins

            Alanna E. Sorenson and Patrick M. Schaeffer

6. Enzyme-Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism

            Rohanah Hussain, Charlotte S Hughes, and Giuliano Siligardi

7. Measuring Small Molecule Binding to Escherichia coli AcrB by Surface Plasmon Resonance

            Steven W. Polyak, Rumana Mowla, and Henrietta Venter

8. Systematic Screening of Viral Entry Inhibitors Using Surface Plasmon Resonance

            Penmetcha K.R. Kumar

9. Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry

            Jan Schejbal, Roman Remínek, and Zdenek Glatz

10. Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA)

            Alanna E. Sorenson and Patrick M. Schaeffer

11. On-Line Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors

            Roman Remínek, Zdenek Glatz, and Wolfgang Thormann

12. Enzymatic Bioautographic Methods

            I. Ayelen Ramallo, Mario O. Salazar, and Ricardo L.E. Furlan

13. High-Throughput Assessment of Metabolism-Induced Toxicity of Compounds on a 384-Pillar Plate

            Soo-Yeon Kang, Kyeong-Nam Yu, Pranav Joshi, and Moo-Yeal Lee

14. Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors

            Abraham Ochoa, Frida Trejo, and Luis F. Olguín

15. Ligand Fishing: An Approach for the Discovery Inhibitors from Complex Biological Mixtures

            Evangelia G. Chronopoulou, Christina Varotsou, Nikolaos Georgakis, Georgios Permetis, Elisavet Ioannou, and Nikolaos E. Labrou

16. HMG-CoA Reductase as Target for Drug Development

            Baskaran Gunasekaran and Mohd Yunus Shukor

17. Lipoxygenases as Targets for Drug Development

            Xiao-Yuan Mao

18. Cholinesterase as a Target for Drug Development in Alzheimer's Disease

            Piyoosh Sharma, Manish Kumar Tripathi, and Sushant Kumar Shrivastava