Nanoparticles in Biology and Medicine: Methods and Protocols

Part I: Synthesis of Nanoparticles and Their Applications in Biology and Medicine

1. Procedures for the Synthesis and Capping of Metal Nanoparticles

            Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, and Miguel José-Yacamán

2. Antimicrobial Applications of Silver Nanoparticles to E. coli Colony Biofilms

            James P. McEvoy, Kayra Genc, Priya Loi, and William J. Walker

3. Elastin-Based Antimicrobial Particles for Delivery of Bioactive Compounds

            Raul Machado, André da Costa, Ana Margarida Pereira, José Carlos Rodriguez-Cabello, and Margarida Casal

4. Biomimetic Lipid Polymer Nanoparticles for Drug Delivery

            Ana Maria Carmona-Ribeiro

5. Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications

            Mirian A.F. Hayashi, Joana D'Arc Campeiro, Lucas Carvalho Porta, Brian Szychowski, Wendel Andrade Alves, Eduardo B. Oliveira, Irina Kerkis, Marie-Christine Daniel, and Richard L. Karpel

6. Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection

            Fan Zhang and Hao-Ying Li

7. Preparation of Hyaluronic Acid-Based Nanoparticles for Macrophage-Targeted MicroRNA Delivery and Transfection

            Neha N. Parayath and Mansoor M. Amiji

8. Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles

            Alexander J. Wilson, Qingyu Zhou, Ian Vargas, Rohun Palekar, Ryan Grabau, Hua Pan, and Samuel A. Wickline

9. Biomedical In Vivo studies with ORMOSIL Nanoparticles Containing Active Agents

            Sona Gandhi and Indrajit Roy

10. Preparation of Spray-Dried Nanoparticles for Efficient Drug Delivery to the Lungs

            Hao-Ying Li and Fan Zhang

11. Synthesis and Evaluation of Airway-Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases

            Neeraj Vij

12. Synthesis and Evaluation of Dendrimers for Autophagy Augmentation and Alleviation of Obstructive Lung Diseases

            Neeraj Vij

13. Synthesis of Poly (2-Hydroxyethyl Methacrylate) (PHEMA)-Based Superparamagnetic Nanoparticles for Biomedical and Pharmaceutical Applications

            Rajesh Saini, Jaya Bajpai, and Anil K. Bajpai

Part II: Focus on the Nanoparticles Derivatization, Bio-Interface, and Nanotoxicity

14. The Molecular Dynamics Simulation of Peptides on Gold Nanosurfaces

            Danilo Roccatano

15. Protein Immobilization on Gold Nanoparticles: Quantitative Analysis

            Evan Decker, Chunsheng Bai, Lauren Nelless, Enrico Ferrari, and Mikhail Soloviev

16. Oriented Immobilization on Gold Nanoparticles of a Recombinant Therapeutic Zymogen

            Elina Dosadina, Celetia Agyeiwaa, William Ferreira, Simon Cutting, Abdullah Jibawi, Enrico Ferrari, and Mikhail Soloviev

17. Directed and Modular Protein Immobilization on Gold and Silver Nanoparticles

            Angela Saccardo, Wenwei Ma, Mikhail Soloviev, and Enrico Ferrari

18. In Vitro Labeling Mesenchymal Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Efficacy and Cytotoxicity

            Jasmin

19. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Color Multiplexing and Application to Chronic Myeloid Leukemia Diagnostics

            Pedro Viana Baptista

20. Assessment of Toxicity of Nanoparticles Using Insects as Biological Models

            Yan Zhou, Yan Chen, Aracely Rocha, Carlos J. Sanchez, and Hong Liang

Part III: Nanoparticles Characterization and Advanced Methods Development

21. Absolute Quantification of Gold Nanoparticles with Femtomolar Accuracy Using Inductively Coupled Plasma Atomic Emission Spectroscopy

            Lee-Anne McCarthy, Andrew Dye, and Enrico Ferrari

22. NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions

            Duncan Griffiths, Pauline Carnell-Morris, and Matthew Wright

23. Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules and Gas Sensor Applications

            Klaus Leifer, Syed Hassan Mujtaba Jafri, and Yuanyuan Han

24. Nanoparticle Characterization through Nano-Impact Electrochemistry: Tools and Methodology Development

            Kevin A. Kirk, Tulashi Luitel, Farideh Hosseini Narouei, and Silvana Andreescu

25. Photostability of Semiconductor Quantum Dots in Response to UV Exposure

            Julian Bailes

26. Gold Nanoclusters, Gold Nanoparticles, and Analytical Techniques for Their Characterization

            Germán Plascencia-Villa, Rubén Mendoza-Cruz, Lourdes Bazán-Díaz, and Miguel José-Yacamán

27. Combining Nanoparticles with Colloidal Bubbles: A Short Review

            Ekaterina Litau

28. Combination of Dark-Field and Confocal Microscopy for the Optical Detection of Silver and Titanium Nanoparticles in Mammalian Cells

            Robert Martin Zucker and William K. Boyes

29. Detection of Silver and TiO₂ Nanoparticles in Cells by Flow Cytometry

            Robert Martin Zucker and William K. Boyes