Cryopreservation and Freeze-Drying Protocols

PART I: Fundamental Aspects And Freezing Technology

1 Principles of Cryopreservation

David E. Pegg

2 Principles of Cryopreservation By Vitrification Gregory M. Fahy and Brian Wowk

3 Modeling and Optimization of Cryopreservation James Benson

4 The Principles of Freeze-Drying Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward

PART II: Methods To Study Freezing and Drying Processes

5. Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells Willem F. Wolkers and Harriëtte Oldenhof

6. Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations

Wendell Q. Sun

7. Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy

Jens O. M. Karlsson

8. Laser Scanning Microscopy in Cryobiology Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann

9. Low Temperature Electron Microscopy Roland A. Fleck

PART III: Cryopreservation Protocols

10 Cryopreservation of Semen From Domestic Livestock Harald Sieme, Harriëtte Oldenhof

11. Cryopreservation of Mammalian Oocytes Victoria Keros, Barry J. Fuller

12. Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts

Juergen Liebermann

13 Efficient Cryopreservation of Human Pluripotent Stem Cells By

Surface-Based Vitrification Julia C. Neubauer, Axel F. Beier, Nils Geijsen, and Heiko Zimmermann

14 Cryopreservation Of Greenshell(TM) Mussel (Perna Canaliculus) Sperm Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. Mcgowan, and

H. Robin Tervit

15 Membrane Modification Strategies for Cryopreservation

Phillip H. Purdy and James K. Graham

16. Sperm Clean-Up and Density Centrifugation Procedures

Harald Sieme and Harriëtte Oldenhof

17. Cryopreservation of Red Blood Cells Johan W. Lagerberg

18. Cord Blood Clinical Processing, Cryopreservation, and Storage

Heidi Elmoazzen and Jelena L. Holovati

19. Directional Freezing For Large Volume Cryopreservation Joseph Saragusty

20 Vitrification Of Heart Valve Tissues Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene,

and Lia H. Campbell

21 Cryopreservation of Plant Cell Lines Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack

22 Writing Standard Operating Procedures (Sops) For Cryostorage Protocols:

Using Shoot Meristem Cryopreservation As An Example Keith Harding and Erica E. Benson

PART IV: Freeze-Drying Protocols

23 Freeze-Drying Of Proteins Baolin Liu, Xinli Zhou

24 Freeze-Drying Of Lactic Acid Bacteria Fernanda Fonseca, Stéphanie Cenard, Stéphanie Passot

25 Freeze-Drying Of Mammalian Sperm Levent Keskintepe, Ali Eroglu

26 Freeze-Drying Of Decellularized Heart Valve Tissues Willem F. Wolkers, Andres Hilfiker